ADC characterization of region-specific response to cerebral perfusion deficit in rats by MRI at 9.4 T.
ADC characterization of region-specific response to cerebral perfusion deficit in rats by MRI at 9.4 T.
复制标题
通过 MRI 在 9.4 T 下对大鼠脑灌注不足的区域特异性反应进行 ADC 表征。
DOI：
10.1002/mrm.10103
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发表时间：
2002
影响因子：
3.3
通讯作者：
Xu,Yan
中科院分区：
文献类型：
--
作者：
Yushmanov,VictorE;Wang,Lei;Liachenko,Serguei;Tang,Pei;Xu,Yan
在大脑中动脉闭塞（MCAO）后6-7 h，通过高场MRI（9.4 T）在体内定量大鼠脑中的区域特异性脑血流量（CBF）和水的表观扩散系数（ADC）。闭塞后，皮质区和杏仁核的平均CBF从约1.5-2 ml/g/min降至0.5 ml/g/min以下，尾壳核降至0.2 ml/g/min以下。某些同侧对侧脑区的CBF也下降了20- 30%。在尾壳核和顶叶皮层，平均ADC从约8 · 10− 4下降到5 · 10− 4 mm 2/s。ADC的相应变化在额叶皮质中较低，在梨状皮质中可忽略不计，这表明ADC降低的灌注阈值对于同一动物的不同脑区可能是不同的。ADC降低的面积与体外脑切片2，3，5-氯化三苯基四氮唑（TTC）染色显示的梗死面积相关性良好。更好地了解ADC和CBF变化与缺血性细胞疾病的联系机制，可能有助于表征组织损伤程度，并制定和评估治疗策略。Magn Reson Med 47：562-570，2002。© 2002 Wiley利斯公司
Region‐specific cerebral blood flow (CBF) and apparent diffusion coefficient (ADC) of water in the rat brain were quantified in vivo by high‐field MRI (9.4 T) for 6–7 h after middle cerebral artery occlusion (MCAO). Upon occlusion, average CBF fell from about 1.5–2 ml/g/min to below 0.5 ml/g/min in cortical areas and the amygdala, and below 0.2 ml/g/min in the caudate putamen. CBF in some of the homologous contralateral areas also decreased by 20–30%. Average ADC decreased from about 8 · 10−4to 5 · 10−4mm2/s in the caudate putamen and parietal cortex. Corresponding changes in ADC were lower in the frontal cortex and negligible in the piriform cortex, suggesting that the perfusion threshold for ADC decrease may be different for different brain regions in the same animal. The area of decreased ADC correlated well with the infarction area revealed by 2,3,5‐triphenyltetrazolium chloride (TTC) staining of brain slices in vitro. A better understanding of the mechanisms linking ADC and CBF changes to ischemic cell disorders may prove useful in characterizing the degree of tissue damage, and in developing and evaluating treatment strategies. Magn Reson Med 47:562–570, 2002. © 2002 Wiley‐Liss, Inc.