Alterations of intestinal serotonin following nanoparticle exposure in embryonic zebrafish.
Alterations of intestinal serotonin following nanoparticle exposure in embryonic zebrafish.
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DOI：
10.1039/c3en00001j
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发表时间：
2014-02-01
期刊：
影响因子：
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通讯作者：
Andreescu S
中科院分区：
文献类型：
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作者：
Ozel RE;Wallace KN;Andreescu S
工程纳米颗粒（NPs）在制造和消费产品中的使用增加，引起了人们对生态系统和活生物体的潜在环境和健康影响的关注。在整个生物体中，最先受到环境NPs暴露影响最严重的器官是皮肤和消化系统。我们研究了两种NPs，镍（Ni）和氧化铜（CuO）对活体水生系统斑马鱼胚胎肠道生理的毒性作用。在胚胎发育的不同阶段，胚胎暴露于不同浓度的Ni和CuO NP。我们使用生理5 -羟色胺（5HT）浓度的变化，通过插入活胚胎的碳纤维微电极电化学测定，来评估NP暴露导致的器官功能障碍。我们发现，暴露于Ni和CuO NPs都会引起生理5HT浓度的变化，这种变化随NPs的类型、暴露时间和浓度以及胚胎暴露的发育阶段而变化。这些数据表明，暴露于NPs可能会改变生物体的发育和生理过程，并为NPs对肠道生理的影响提供证据。
The increased use of engineered nanoparticles (NPs) in manufacturing and consumer products raises concerns about the potential environmental and health implications on the ecosystem and living organisms. Organs initially and more heavily affected by environmental NPs exposure in whole organisms are the skin and digestive system. We investigate the toxic effect of two types of NPs, nickel (Ni) and copper oxide (CuO), on the physiology of the intestine of a living aquatic system, zebrafish embryos. Embryos were exposed to a range of Ni and CuO NP concentrations at different stages of embryonic development. We use changes in the physiological serotonin (5HT) concentrations, determined electrochemically with carbon fiber microelectrodes inserted in the live embryo, to assess this organ dysfunction due to NP exposure. We find that exposure to both Ni and CuO NPs induces changes in the physiological 5HT concentration that varies with the type, exposure period and concentration of NPs, as well as with the developmental stage during which the embryo is exposed. These data suggest that exposure to NPs might alter development and physiological processes in living organisms and provide evidence of the effect of NPs on the physiology of the intestine.