Stability enhancement and circular dichroism spectral anomaly as an indication of non-covalent interactions in histidine- and tyrosine-containing ternary copper(II)—amino acid systems
Stability enhancement and circular dichroism spectral anomaly as an indication of non-covalent interactions in histidine- and tyrosine-containing ternary copper(II)—amino acid systems
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稳定性增强和圆二色性光谱异常作为含组氨酸和酪氨酸三元铜（II）-氨基酸系统中非共价相互作用的指示
DOI：
10.1016/s0020-1693(00)88304-8
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发表时间：
1985
影响因子：
2.8
通讯作者：
A. Odani
中科院分区：
文献类型：
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作者：
O. Yamauchi;A. Odani
对含组氨酸和酪氨酸的三元铜（II）配合物Cu（A）（L-B）（A为L-His、D-His或L-Tyr，B为Lys、Tyr、Trp、Phe、Ala、瓦尔、Arg、Glu、Asn、Gln、Ser或Thr）进行了可见吸收光谱、圆二色光谱和电位滴定研究，以研究配合物中配体间的相互作用。虽然在不存在侧链相互作用的情况下，d-d区域中的CD光谱幅度是加和的，并且可以根据涉及DL-A或DL-B的三元体系的幅度进行估计，但对于Cu（L-His）（L-B）（B= LysH、Tyr、Trp或Phe）和Cu（L-Tyr）（L-Trp），观察到与加和性的偏差。根据在25 ℃和I= 0.1M（KNO 3）下测定的稳定常数，计算出下列假设平衡的平衡常数K很大（0.14-0.60）用于形成Cu（L-/D-His）（L-B）（B= Tyr或Trp）和Cu（D-His）（L-Phe），Cu（en）（L-Ala）作为标准品：C u（A）（L-A l a）+ C u（en）（B）<$K C u（A）（B）+ C u（en）（L-A l a）正值表示由于His的咪唑环和L-B的芳族侧链之间的堆叠而产生的稳定性。Cu（L-His）（L-LysH）和Cu（L-His）L-Trp）的CD光谱的溶剂依赖性进一步支持了分子内静电和疏水相互作用的存在。
Visible absorption and CD spectral and potentiometric studies on the His-and Tyr-containing ternary copper (II) complexes Cu (A)(L-B), where A refers to L-His, D-His, or L-Tyr and B to Lys, Tyr, Trp, Phe, Ala, Val, Arg, Glu, Asn, Gln, Ser, or Thr, were made to study ligand-ligand interactions in the complexes. While the CD spectral magnitudes in the d—d region are additive in the absence of side chain interactions and can be estimated from the magnitudes for the ternary systems involving DL-A or DL-B, deviation from the additivity was observed for Cu (L-His)(L-B)(B= LysH, Tyr, Trp, or Phe) and Cu (L-Tyr)(L-Trp). From the stability constants determined at 25° C and I= 0.1 M (KNO 3), the equilibrium constants, K, for the following hypothetical equilibria were calculated to be large (0.14–0.60) for formation of Cu (L-/D-His)(L-B)(B= Tyr or Trp) and Cu (D-His)(L-Phe) with Cu (en)(L-Ala) as standard: C u (A)(L− A l a)+ C u (e n)(L− b)⇌ K C u (A)(L− B)+ C u (e n)(L− A l a) The positive values indicate the stabilization due to the stacking between the imidazole ring of His and the aromatic side chain of L-B. Solvent dependence of the CD spectra for Cu (L-His)(L-LysH) and Cu (L-His) L-Trp) further supported the existence of the intramolecular electrostatic and hydrophobic interactions.
Akira Odani：“氨基酸侧链磷酸化对三元铜 (II) 配合物形成的影响。磷酸酯与精氨酸或赖氨酸侧基之间的分子内静电配体-配体相互作用”
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