Metabolomic responses of sea cucumber Apostichopus japonicus to thermal stresses
Metabolomic responses of sea cucumber Apostichopus japonicus to thermal stresses
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DOI：
10.1016/j.aquaculture.2014.10.023
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发表时间：
2015
期刊：
影响因子：
4.5
通讯作者：
Y. Shao;Chenghua Li;Xiaocong Chen;Pengjuan Zhang;Ye Li;Taiwu Li;Jinbo Jiang
中科院分区：
文献类型：
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作者：
Y. Shao;Chenghua Li;Xiaocong Chen;Pengjuan Zhang;Ye Li;Taiwu Li;Jinbo Jiang
水温升高通常被认为是刺参养殖中最普遍和最有问题的压力源之一。然而，到目前为止，人们对FA的内在机制知之甚少。日本人在新陈代谢水平上应对气温上升。在本研究中，研究了OFA肌肉的代谢反应。通过基于~1H核磁共振的代谢组学，日本血吸虫在20℃和25℃两个热点。结果表明，暴露0天和3天后，两种温度都干扰了能量代谢，并诱导了以苏氨酸、丙氨酸、精氨酸、谷氨酸、酪氨酸、组氨酸、甘氨酸、富马酸、葡萄糖和AMP等代谢生物标志物为基础的渗透调节，降低了ATP、天冬氨酸、次牛磺酸和胆碱。在热应激7天时，几种代谢物也发生了类似的变化，包括20℃时谷氨酰胺、甘氨酸、葡萄糖、AMP、酪氨酸和组氨酸的增加以及ATP的降低。令人惊讶的是，在25℃时，大量氨基酸(缬氨酸、亮氨酸、异亮氨酸、苏氨酸、精氨酸、谷氨酸、苯丙氨酸)显著下降。此外，还观察了HSP70和ATP合成酶的mRNA表达水平以及SOD活性和MDA含量，并在不同的暴露条件下其表达水平发生了显著变化。我们目前的结果为我们对NA的热应力效应提供了新的见解。日本血吸虫的代谢反应。
Elevated water temperature is generally considered as one of the most widespread and problematic stressors onApostichopus japonicusfarming. To date, however, little is known about the intrinsic mechanisms ofA. japonicusto cope with the rising temperature at the metabolic level. In the present study, the metabolomic responses were characterized in the muscles ofA. japonicusat two thermal points, 20 °C and 25 °C by1H NMR-based metabolomics. The results showed that both temperatures disturbed energy metabolism and induced osmotic regulation based on the metabolic biomarkers such as elevated threonine, alanine, arginine, glutamate, tyrosine, histidine, glycine, fumarate, glucose, and AMP and decreased ATP, aspartate, hypotaurine, and choline after exposure for 0 days and 3 days. At 7 days of thermal-stress, several metabolites were similarly altered including the increased glutamine, glycine, glucose, AMP, tyrosine, and histidine and the decreased ATP at 20 °C. Surprisingly, a large amount of amino acids (valine, leucine, isoleucine, threonine, arginine, glutamate, phenylalanine) markedly declined at 25 °C. In addition, the mRNA expression levels of HSP70 and ATP synthase as well as SOD activity and MDA content were also investigated, and their expression levels were significantly altered at different exposure conditions. Our present results provided new insights into thermal-stressed effects onA. japonicusat metabolic responses.