Estrogen-induced progesterone receptor in the Syrian hamster kidney. I. Modulation by antiestrogens and androgens.
Estrogen-induced progesterone receptor in the Syrian hamster kidney. I. Modulation by antiestrogens and androgens.
复制标题
叙利亚仓鼠肾脏中雌激素诱导的孕激素受体。
DOI：
10.1210/endo-103-6-2119
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发表时间：
1978
期刊：
影响因子：
4.8
通讯作者：
J. Li
中科院分区：
文献类型：
--
作者：
S. Li;J. Li
我们之前的研究证明了雌激素诱导和依赖性仓鼠肾癌中存在特定的孕酮受体，并提供了雌激素治疗在仓鼠肾细胞质中诱导4S孕酮结合成分的证据。后一个发现代表了迄今为止在雌激素肾肿瘤发生诱导期间报道的第一个生理变化。目前的研究表明，雌激素引发的仓鼠肾脏中特异性孕酮结合最早明显增加发生在激素治疗 3 周后。通过竞争性结合测定和蔗糖梯度离心确定雌激素化仓鼠肾胞质中黄体酮受体的激素特异性。这些数据表明孕激素受体是特异性的，因为雌激素、雄激素和大多数孕激素代谢物在过量 100 倍时不会明显竞争该受体，而未标记的孕激素显着抑制相应的结合。
Our previous studies demonstrated a specific progesterone receptor in the estrogen-induced and -dependent hamster renal carcinoma and provided evidence that estrogen treatment induces a 4S progesterone- binding component in renal cytosol of the hamster. This latter finding represented the first physiological change thus far reported during the induction period of estrogen renal tumorigenesis. The present studies indicated that the earliest appreciable increase in specific progesterone binding in kidneys of estrogen-primed hamsters occurred after 3 weeks of hormone treatment. The hormone specificity of the progesterone receptor in renal cytosol of estrogenized hamsters was ascertained by competitive binding assay and sucrose gradient centrifugation. These data indicate that the progesterone receptor is specific, since estrogens, androgens, and most progesterone metabolites did not compete appreciably for this receptor at 100-fold excess, whereas unlabeled progesterone markedly inhibited the binding at corr...