Correlation of antithyroglobulin and antithyroid-peroxidase antibody profiles with clinical and ultrasound characteristics of chronic thyroiditis
Correlation of antithyroglobulin and antithyroid-peroxidase antibody profiles with clinical and ultrasound characteristics of chronic thyroiditis
复制标题
DOI：
10.1089/thy.1998.8.1101
复制
发表时间：
1998-12-01
期刊：
影响因子：
6.6
通讯作者：
Hosoya, T
中科院分区：
文献类型：
--
作者：
Takamatsu, J;Yoshida, S;Hosoya, T
根据特定的选择标准选择慢性甲状腺炎患者进行研究，并将酶联免疫吸附试验（ELISA）方法与血凝法进行比较。ELISA检测含有抗甲状腺球蛋白（TG）抗体和抗甲状腺过氧化物酶（PO）抗体，后者采用重组人TPO抗体。437例慢性甲状腺炎患者分为四组；两种抗体316例（TG + PO组），单独TG抗体75例（TG组），单独PO抗体10例（PO组），无两种抗体36例（N组）。血球凝集试验结果显示抗体谱差异很大：基于ELISA的PO组仅出现2%，而基于血球凝集试验的单独微粒体抗体组出现39%。随后，在四组患者中进行了疾病特征的研究，包括年龄、性别、家族易感性、甲状腺功能和腺体形态，这些研究基于ELISA谱，而不是血凝结果。TG + PO和TG的亚组来自随机选择的患者（每个亚组中有40名患者）。结果显示，PO组甲状腺体积较小（25 +/- 16 mL，平均+/- SD），回声正常，甲状腺功能减退患病率50%。TG组甲状腺体积较大（57 +/- 42 mL），常伴有小结节形成（53%），但甲状腺功能减退发生率较低（23%）。综上所述，与血凝法相比，ELISA检测慢性甲状腺炎具有更高的敏感性和特异性。ELISA图谱与甲状腺超声检查的相关性检测出亚组之间的细微差异，这可能解释了甲状腺形态和甲状腺功能减退患病率的差异：PO组回声回声正常，但甲状腺功能受损的发生率高，而TG组小结节与甲状腺功能减退的发生率较低相关。
Patients with chronic thyroiditis were selected for study according to specific selection criteria, and comparisons of an enzyme-linked immunosorbent assay (ELISA) method to a hemagglutination method were performed. The ELISA assays contained antithyroglobulin (TG) antibody and antithyroid-peroxidase (PO) antibody, the latter using recombinant human TPO. Four groups were established from 437 patients with chronic thyroiditis; 316 with both antibodies (TG + PO group), 75 with TG antibody alone (TG group), 10 with PO antibody alone (PO group), and 36 with no such antibodies (N group). The hemagglutination assay resulted in much different antibody profiles: PO group based on ELISA occurred in only 2%, whereas a group with microsome antibody alone based on hemagglutination assay occurred in 39%. Subsequently, studies of disease characteristics including age, gender, familial predisposition, thyroid function, and morphology of the gland were performed among the four groups of patients based on ELISA profiles but not on hemagglutination results. The subgroups of TG + PO and TG were derived from randomly selected patients (40 out of a much larger number of patients in each). The results showed that the PO group had smaller thyroid volume (25 +/- 16 mL, mean +/- SD) with normal echogenicity, and 50% prevalence of hypothyroidism. The TG group had larger thyroid volume (57 +/- 42 mL) with frequent association of small nodular formation (53%) but less frequent hypothyroidism (23%). In summary, compared to the hemagglutination method, the ELISA was noted to have both a higher sensitivity and specificity for detection of chronic thyroiditis. Correlation of ELISA profiles with ultrasonography of the thyroid gland detected subtle differences in subgroups that may account for differences in thyroid gland morphology and prevalence of hypothyroidism: the PO group has normal echogenicity but high incidence of impaired thyroid function, whereas in the TG group small nodules were associated with a lesser incidence of hypothyroidism.