Xenogeneic and endogenous spermatogenesis following transplantation of rat germ cells into testes of immunocompetent mice.
Xenogeneic and endogenous spermatogenesis following transplantation of rat germ cells into testes of immunocompetent mice.
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DOI：
10.1071/rd10349
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发表时间：
2012-02
期刊：
影响因子：
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通讯作者：
N. Qu;M. Naito;Jun Li;H. Terayama;S. Hirai;M. Itoh
中科院分区：
文献类型：
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作者：
N. Qu;M. Naito;Jun Li;H. Terayama;S. Hirai;M. Itoh
精原干细胞（spermatogonialstemcells，SSCs）是精子发生的基础，具有自我更新和分化后代形成精子的能力。研究表明，大鼠精原干细胞移植后，在先天性免疫缺陷小鼠的曲细精管中可以发生大鼠精子发生。然而，睾丸通常被视为免疫特权部位，因为生殖细胞上的自身免疫原性抗原通常不会原位引发免疫应答。在本研究中，我们试图将大鼠精原干细胞移植到免疫功能正常的小鼠后，通过白消安消耗自己的生殖细胞。结果表明，部分移植的精原干细胞在受体小鼠睾丸中可发生完整的精子生成，28只受体小鼠附睾中有7只可检测到大鼠精子。无论是否同时存在大鼠精子，在受体小鼠的所有28个附睾中也观察到小鼠精子的显著增加。这些结果表明，移植的大鼠精原干细胞可以耐受在免疫活性小鼠的睾丸和大鼠精原干细胞的移植刺激受体小鼠的内源性精子发生。
Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis, and are characterised by their ability to self-renew and to produce differentiated progeny that form spermatozoa. It has been demonstrated that rat spermatogenesis can occur in the seminiferous tubules of congenitally immunodeficient recipient mice after transplantation of rat SSCs. However, the testis is often viewed as an immune-privileged site in that autoimmunogenic antigens on germ cells do not normally elicit an immune response in situ. In the present study, we tried to transplant rat SSCs into immunocompetent mice after depletion of their own germ cells by means of busulfan. The results showed that some transplanted SSCs could undergo complete spermatogenesis in recipient mouse testes, the rat spermatozoa being detected in 7 of 28 recipient epididymides. A significant increase in mouse spermatozoa was also noted in all 28 epididymides of recipient mice regardless of whether rat spermatozoa were concurrently present or not. These results suggest that transplanted rat SSCs can be tolerated in the testes of immunocompetent mice and that the transplantation of rat SSCs stimulates endogenous spermatogenesis in the recipient mice.