Induced Germinal Center B Cell Culture System
Induced Germinal Center B Cell Culture System
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DOI：
10.21769/bioprotoc.3163
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发表时间：
2019-02-20
期刊：
影响因子：
0.8
通讯作者：
Kitamura, Daisuke
中科院分区：
文献类型：
--
作者：
Haniuda, Kei;Kitamura, Daisuke
生发中心 (GC) 是 B 细胞进行克隆扩增、基于亲和力的选择以及分化为记忆 B 细胞或浆细胞的场所。阐明动态 GC B 细胞成熟和分化的调节机制一直很困难，部分原因是体内 GC B 细胞的实验操作受到限制，并且没有类似于 GC 中 B 细胞反应的体外系统。在这里，我们描述了名为“诱导 GC B (iGB) 培养系统”的培养系统的协议，该系统可以诱导表现出 GC B 细胞样表型的 B 细胞大量扩增，从而模拟 GC 反应。该方案可用于阐明 GC B 细胞分化的分子机制。
The germinal center (GC) is the site where B cells undergo clonal expansion, affinity-based selection, and differentiation into memory B cells or plasma cells. It has been difficult to elucidate regulatory mechanisms for the dynamic GC B cell maturation and differentiation, partly because experimental manipulation of GC B cells in vivo has been limited and no in vitro system has been available that resembles B cell reaction in GC. Here we describe the protocol for a culture system named "induced GC B (iGB) culture system" which can induce massive expansion of B cells that exhibit GC B cell-like phenotype, and thus it mimics the GC reaction. This protocol can be useful to elucidate the molecular mechanisms of GC B cell differentiation.