Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of tenofovir and emtricitabine in human plasma and its application to a bioequivalence study
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of tenofovir and emtricitabine in human plasma and its application to a bioequivalence study
复制标题
DOI：
10.1016/j.jpba.2008.07.022
复制
发表时间：
2008-11-04
影响因子：
3.4
通讯作者：
Parekh, Sagar A.
中科院分区：
文献类型：
--
作者：
Gomes, Noel A.;Vaidya, Vikas V.;Parekh, Sagar A.
A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for simultaneous quantification of Tenofovir (TEN) and Erntricitabine (EMT) in human plasma using Chromolith Speed Rod RP18. The mass transition ion-pair has been followed as m/Z 288.10 -> 176.10 for TEN, m/Z 248.20 -> 130.20 for EMT and m/z 230.10 -> 112.10 for Lamivudine (LAM). The method involves solid phase extraction from plasma, simple isocratic chromatographic conditions and mass spectrometric detection using an API 5000 instrument that enables detection at nanogram levels. Lamivudine was used as the internal standard. The proposed method has been validated with a linear range of 10-600 ng/ml for TEN and 25-2500 ng/ml for EMT. The intrarun and interrun precision values are within 12.0% for TEN and 15.6% for EMT at their respective LOQ levels. The overall recoveries for TEN and EMT were 84.3% and 68.5%, respectively. Total elution time was as low as 2 min. (c) 2008 Elsevier B.V. All rights reserved.
