Electron spin resonance studies on deuterated nitroxyl spin probes used in Overhauser-enhanced magnetic resonance imaging
Electron spin resonance studies on deuterated nitroxyl spin probes used in Overhauser-enhanced magnetic resonance imaging
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DOI：
10.1002/mrc.4576
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发表时间：
2017-08-01
影响因子：
2
通讯作者：
Benial, A. Milton Franklin
中科院分区：
文献类型：
--
作者：
Jebaraj, D. David;Utsumi, Hideo;Benial, A. Milton Franklin
利用x波段电子自旋共振谱仪对2mm浓度n -14标记和n -15标记的3-氨基甲酰-2,2,5,5-四甲基吡咯烷-1-氧、3-羧基-2,2,5,5-四甲基吡咯烷-1-氧、3-甲氧基羰基-2,2,5,5-四甲基吡咯烷-1-氧及其氘化的硝基自由基进行了电子自旋共振研究。进行了电子自旋共振线形分析。估计了电子自旋共振的线宽、洛伦兹分量、信号强度比、旋转相关时间、超精细耦合常数和g因子等参数。与未氘化的硝基自由基相比，氘化后的硝基自由基线宽窄，洛伦兹组分增加。对所有的硝基自由基均观察到动态核极化因子。H-2标记后，n -14标记和n -15标记的硝基自由基的动态核极化因子分别增加了约70%和40%。信号强度比和g值表明了硝基自由基在纯水中的各向同性。因此，氘化的硝基自由基是体内/体外电子自旋共振和奥弗豪斯增强磁共振成像方式的合适自旋探针。版权所有：John Wiley & Sons， Ltd。
The electron spin resonance studies were carried out for 2mm concentration of N-14-labeled and N-15-labeled 3-carbamoyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl, 3-carboxy-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl, 3-methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl and their deuterated nitroxyl radicals using X-band electron spin resonance spectrometer. The electron spin resonance line shape analysis was carried out. The electron spin resonance parameters such as linewidth, Lorentzian component, signal intensity ratio, rotational correlation time, hyperfine coupling constant and g-factor were estimated. The deuterated nitroxyl radicals have narrow linewidth and an increase in Lorentzian component, compared with undeuterated nitroxyl radicals. The dynamic nuclear polarization factor was observed for all nitroxyl radicals. Upon H-2 labeling, about 70% and 40% increase in dynamic nuclear polarization factor were observed for N-14-labeled and N-15-labeled nitroxyl radicals, respectively. The signal intensity ratio and g-value indicate the isotropic nature of the nitroxyl radicals in pure water. Therefore, the deuterated nitroxyl radicals are suitable spin probes for in vivo/in vitro electron spin resonance and Overhauser-enhanced magnetic resonance imaging modalities. Copyright (C) 2017 John Wiley & Sons, Ltd.