Association of α2-HS glycoprotein (AHSG, fetuin-A) polymorphism with AHSG and phosphate serum levels
Association of α2-HS glycoprotein (AHSG, fetuin-A) polymorphism with AHSG and phosphate serum levels
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DOI：
10.1007/s00439-004-1222-7
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发表时间：
2005-02-01
期刊：
影响因子：
5.3
通讯作者：
Umetsu, K
中科院分区：
文献类型：
--
作者：
Osawa, M;Tian, W;Umetsu, K
Alpha2-HS 糖蛋白 (AHSG)，也称为胎球蛋白-A，是一种血浆蛋白，与磷酸钙具有高亲和力相互作用，可防止异位血管钙化。本研究试图评估 AHSG 多态性与 AHSG 血清水平和钙相关参数之间的关系。通过定量火箭免疫电泳测量无关个体的 AHSG 水平，AHSG1 纯合子 (n = 99)、杂合子 (n = 55) 和 AHSG2 纯合子 (n = 22) 三种主要基因型的 AHSG 水平分别为 581 +/- 38、542 +/- 31 和 494 +/- 23 mg/l（差异显着：P< 0.001）。因此，循环 AHSG 水平受到遗传多态性以及 AHSG2 等位基因加性减少的影响。简单和多元回归模型的统计分析显示，AHSG 水平与总钙、白蛋白校正总钙和离子钙的血清值之间没有关联。然而，AHSG 水平与游离磷酸盐水平呈显着负相关（P<0.001），表明 AHSG 是血清磷酸盐的新决定因素。 AHSG 多态性可归因于 AHSG 和磷酸盐血清水平的遗传变异，这可能会影响骨骼发育和血管钙化等慢性疾病。
Alpha2-HS glycoprotein (AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. This investigation has attempted to evaluate the relationship between AHSG polymorphism and serum levels of AHSG and calcium-related parameters. AHSG levels in unrelated individuals were measured by quantitative rocket immunoelectrophoresis and were 581 +/- 38, 542 +/- 31, and 494 +/- 23 mg/l for three major genotypes of AHSG1 homozygotes (n = 99), heterozygotes (n = 55), and AHSG2 homozygotes ( n = 22), respectively (differences were significant: P< 0.001). The circulating AHSG level was therefore influenced by the genetic polymorphism with the additive reduction in the AHSG2 allele. Statistical analysis of simple and multiple regression models revealed no associations between AHSG levels and serum values of total calcium, albumin-corrected total calcium, and ionized calcium. However, the AHSG levels demonstrated a significant negative correlation with free phosphate levels ( P< 0.001), indicating that AHSG is a novel determinant of serum phosphate. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.