Prediction of a peptidome for the western tarnished plant bug Lygus hesperus
Prediction of a peptidome for the western tarnished plant bug Lygus hesperus
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DOI：
10.1016/j.ygcen.2016.10.008
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发表时间：
2017-03-01
影响因子：
2.7
通讯作者：
Tassone, Erica E.
中科院分区：
文献类型：
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作者：
Christie, Andrew E.;Hull, J. Joe;Tassone, Erica E.
许多控制害虫的策略需要了解它们的激素信号传导剂，肽是这些分子中最大和最多样化的一类。豆荚草盲蝽是一种特别令人关注的害虫物种，因为它对多种商业上重要的植物作物造成重大损害。目前，对L.赫斯珀勒斯。在这里，转录组学数据用于预测L hesperus的肽组。五十三湖鉴定了编码肽前体的hesperus转录物，其中一个子集通过PCR扩增用于序列验证。从这些成绩单推导出的蛋白质允许预测的119个序列的肽组L hesperus。预测的肽包括allatostatin A、allatostatin B（AST-B）、allatostatin C、allatotropin、囊体激素、CCHamide、corazonin、甲壳动物心脏活性肽、甲壳动物高血糖激素/离子转运肽、利尿激素31、GSEFLamide、胰岛素样肽、肌抑制素、neuroparsin、神经肽F、orcoldnin、orcomyotropin、pyrokinin、短神经肽F、SIFamide、磺胺激肽和速激肽相关肽的同种型。值得注意的是AST-B的几种同种型具有-WX（6）W酰胺羧基末端而不是常规的-WX（6）W酰胺（例如，KWQDMQNPGWamide）、以-SARGF amide而不是-TARGF amide结尾的促生长素（GLKNGPLNSARGF amide）、以-GTEFL amide结尾的GSEFL amide（TVGTEFL amide）、具有PMDEIDR-而不是NFDEIDR-氨基末端的几种虎激肽（例如，PMDEIDRAGFTEIFV），和8个而不是12个氨基酸长的SIFamide同种型（PPFNGSIFamide）。总的来说，这里预测的L hesperus peptidome提供了一个资源，启动生理调查的肽能信号在这个物种中，包括针对这种农业害虫的生物防治的研究。(C)2016 Elsevier Inc. All rights reserved.
Many strategies for controlling insect pests require an understanding of their hormonal signaling agents, peptides being the largest and most diverse single class of these molecules. Lygus hesperus is a pest species of particular concern, as it is responsible for significant damage to a wide variety of commercially important plant crops. At present, little is known about the peptide hormones of L. hesperus. Here, transcriptomic data were used to predict a peptidome for L hesperus. Fifty-three L. hesperus transcripts encoding peptide precursors were identified, with a subset amplified by PCR for sequence verification. The proteins deduced from these transcripts allowed for the prediction of a 119-sequence peptidome for L hesperus. The predicted peptides include isoforms of allatostatin A, allatostatin B (AST-B), allatostatin C, allatotropin, bursicon, CCHamide, corazonin, crustacean cardioactive peptide, crustacean hyperglycemic hormone/ion transport peptide, diuretic hormone 31, GSEFLamide, insulin-like peptide, myosuppressin, neuroparsin, neuropeptide F, orcoldnin, orcomyotropin, pyrokinin, short neuropeptide F, SlFamide, sulfakinin and tachykinin-related peptide. Of note were several isoforms of AST-B that possess -WX7Wamide carboxyl-termini rather than the stereotypical-WX(6)Wamide (e.g., KWQDMQNPGWamide), an allatotropin ending in-SARGFamide rather than-TARGFamide (GLKNGPLNSARGFamide), a GSEFLamide ending in-GTEFLamide (TVGTEFLamide), several orcokinins with PMDEIDR-rather than NFDEIDR- amino-termini (e.g., PMDEIDRAGFTEIFV), and an eight rather than 12 amino acid long isoform of SlFamide (PPFNGSIFamide). Collectively, the L hesperus peptidome predicted here provides a resource for initiating physiological investigations of peptidergic signaling in this species, including studies directed at the biological control of this agricultural pest. (C) 2016 Elsevier Inc. All rights reserved.