Thc6 protein, isolated from Trichoderma harzianum, can induce maize defense response against Curvularia lunata
Thc6 protein, isolated from Trichoderma harzianum, can induce maize defense response against Curvularia lunata
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从哈茨木霉中分离出的 Thc6 蛋白可诱导玉米针对新月弯孢菌的防御反应
DOI：
10.1002/jobm.201300814
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发表时间：
2015-05-01
影响因子：
3.1
通讯作者：
Chen, Jie
中科院分区：
文献类型：
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作者：
Fan, Lili;Fu, Kehe;Chen, Jie
从根癌农杆菌介导法构建的450株哈茨木霉突变体中筛选到突变体T66。与野生型（WT）菌株相比，T66包衣的玉米种子更易感染弯孢菌。用T66和WT处理的玉米的病情指数分别为62.5%和42.1%。进一步的研究表明，T-DNA已经插入到一个基因的ORF中，这导致了WT和T66之间的功能差异。该基因被克隆并命名为Thc 6，其编码一种新的327个氨基酸的蛋白。为了研究其功能，我们获得了Thc 6的敲除、互补和过表达突变体。攻毒试验表明，Thc 6过表达突变体能降低玉米自交系黄早4号对玉米叶斑病的病情指数。lunata）。同时，Thc 6突变体对玉米自交系黄早4号的抗病性也有影响。通过增强茉莉酸响应基因的表达激活lunata。液相色谱-质谱（LC-MS）数据进一步证实了诱导玉米中茉莉酸的浓度与防御相关基因的表达水平呈平行变化趋势。因此，Thc 6基因可能参与了玉米自交系黄早4号对白叶枯病的诱导抗性。lunata感染通过茉莉酸依赖途径。
Mutant T66 was isolated from 450 mutants (constructed with Agrobacterium tumefaciens-mediated transformation method) of Trichoderma harzianum. Maize seeds coated with T66 were more susceptible to Curvularia lunata when compared with those coated with wild-type (WT) strain. The disease index of maize treated with T66 and WT were 62.5 and 42.1%, respectively. Further research showed T-DNA has inserted into the ORF of one gene, which resulted in the functional difference between WT and T66. The gene was cloned and named Thc6, which encodes a novel 327 amino acid protein. To investigate its function, we obtained knockout, complementation, and overexpression mutants of Thc6. Challenge inoculation studies suggested that the Thc6 overexpression mutant can reduce the disease index of maize inbred line Huangzao 4 against the leaf spot pathogen (C. lunata). Meanwhile, The Thc6 mutants were found to affect the resistance of maize inbred line Huangzao 4 against C. lunata by enhancing the activation of jasmonate-responsive genes expression. Liquid chromatography-mass spectrometry (LC-MS) data further confirmed that the concentration of jasmonate in the induced maize exhibits a parallel change tendency with the expression level of defense-related genes. Hence, the Thc6 gene could be participated in the induced resistance of maize inbred line Huangzao 4 against C. lunata infection through a jasmonic acid-dependent pathway.