In situ characterization of mononuclear cells in human chronic marginal periodontitis using monoclonal antibodies.
In situ characterization of mononuclear cells in human chronic marginal periodontitis using monoclonal antibodies.
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使用单克隆抗体对人类慢性边缘性牙周炎中的单核细胞进行原位表征。
DOI：
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发表时间：
1986
影响因子：
3.5
通讯作者：
T. Kristopfersen
中科院分区：
文献类型：
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作者：
A. C. Johannessen;R. Nilsen;G. E. Knudsen;T. Kristopfersen
应用间接免疫荧光技术，对25例成人慢性边缘牙周炎患者的丙酮固定冰冻切片进行了检测。B淋巴细胞(Leu-12阳性)的数量在不同标本之间差异很大，通常在袋上皮(PE)下细胞浸润物的最顶端出现最多。所有标本均可见不同数量的T淋巴细胞(OKT3阳性)。PE下浸润细胞中辅助性T细胞(OKT4a阳性)多于T抑制/细胞毒细胞(OKT8阳性)(OKT4a/OKT8=1.13)。这些细胞类型在口腔牙龈上皮(OGE)下的分布更为均匀。在OGE内可见朗格汉斯细胞，偶见邻近OGE。炎性细胞浸润和OGE下方结缔组织乳头内可见散在分布的巨噬细胞(Leu-M3或OK Ia1阳性)。与OK Ia1反应的HL A-DR抗原存在于OGE及其邻近OKT 6阳性细胞对应的细胞上，也存在于PE下的炎性细胞和血管周围浸润物中。在一些标本中，还发现人类白细胞抗原-DR与OGE外部的角质形成细胞相关。偶见NK细胞(Leu-7阳性)定位于OGE内及邻近OGE。不同标本之间以及同一标本不同切片上的各种单个核细胞的数量和分布有相当大的差异。
Acetone fixed cryostat sections from 25 patients with adult chronic marginal periodontitis were characterized using an indirect immunofluorescence technique with monoclonal antibodies. The amount of B lymphocytes (Leu-12 positive) varied considerably between the specimens and were usually seen in largest numbers in the most apical parts of the cellular infiltrates beneath the pocket epithelium (PE). Varying amounts of T lymphocytes (OKT 3 positive) were demonstrated in all specimens. The amount of T helper cells (OKT 4a positive) exceeded that of T suppressor/cytotoxic cells (OKT 8 positive) in the cellular infiltrates beneath the PE (OKT 4a/ OKT 8 =1.13). There was a more even distribution of these cell types beneath the oral gingival epithelium (OGE). Langerhans cells were observed within and occasionally subjacent to the OGE. Scattered macrophages (Leu-M3 or OK Ia 1 positive) were observed in the inflammatory cell infiltrates and on the connective tissue papillae beneath the OGE. HLA-DR antigen reacting with OK Ia 1 was present on cells corresponding to OKT 6 positive cells in the OGE and subjacent to the OGE as well as in the inflammatory cell infiltrates beneath the PE and in the perivascular infiltrates. In some specimens HLA-DR antigen was also found to be associated with keratinocytes in the outer parts of the OGE. Occasional NK cells (Leu-7 positive) were localized inside and subjacent to the OGE. There was a considerable variation with respect to the number and distribution of the various mononuclear cells between specimens and from section to section from the same specimen.
单克隆抗人单核细胞抗体 OKM1 和 OKM5 具有独特的组织分布，包括与血管内皮的不同反应性。
DOI：
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发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Knowles2nd,DM;Tolidjian,B;Marboe,C;D'Agati,V;Grimes,M;Chess,L
通讯作者：
Chess,L
DOI：
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发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Dimitriu-Bona,A;Burmester,GR;Waters,SJ;Winchester,RJ
通讯作者：
Winchester,RJ
通过单克隆抗体 (HNK-1) 识别的人类 NK 和 K 细胞的分化抗原。
DOI：
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发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Abo,T;Balch,CM
通讯作者：
Balch,CM