Arabidopsis Regeneration from Multiple Tissues Occurs via a Root Development Pathway
Arabidopsis Regeneration from Multiple Tissues Occurs via a Root Development Pathway
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DOI：
10.1016/j.devcel.2010.02.004
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发表时间：
2010-03-16
影响因子：
11.8
通讯作者：
Meyerowitz, Elliot M.
中科院分区：
文献类型：
--
作者：
Sugimoto, Kaoru;Jiao, Yuling;Meyerowitz, Elliot M.
与大多数动物细胞不同，植物细胞在适当培养时可以很容易地从各种器官再生新组织。为不同植物细胞提供显著再生能力的共同元素在很大程度上仍然是未知的。在这里，我们描述了拟南芥在体外再生的初始过程中，一个多能细胞团被称为愈伤组织诱导。我们证明，愈伤组织类似于根尖分生组织，即使它是来自气生器官，如花瓣，这清楚地表明，愈伤组织的形成是不是一个简单的重编程过程中向后到一个未分化的状态，广泛认为。此外，根、子叶和花瓣中的愈伤组织形成在不能侧根起始的突变体植物中被阻断。因此，侧根发育程序的异位激活似乎是多器官愈伤组织形成的常见机制。
Unlike most animal cells, plant cells can easily regenerate new tissues from a wide variety of organs when properly cultured. The common elements that provide varied plant cells with their remarkable regeneration ability are still largely unknown. Here we describe the initial process of Arabidopsis in vitro regeneration, where a pluripotent cell mass termed callus is induced. We demonstrate that callus resembles the tip of a root meristem, even if it is derived from aerial organs such as petals, which clearly shows that callus formation is not a simple reprogramming process backward to an undifferentiated state as widely believed. Furthermore, callus formation in roots, cotyledons, and petals is blocked in mutant plants incapable of lateral root initiation. It thus appears that the ectopic activation of a lateral root development program is a common mechanism in callus formation from multiple organs.