Cultured schwann cells constitutively express the myelin protein P-0
Cultured schwann cells constitutively express the myelin protein P-0
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DOI：
10.1016/s0896-6273(00)80049-5
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发表时间：
1996-02-01
期刊：
影响因子：
16.2
通讯作者：
Mudge, AW
中科院分区：
文献类型：
--
作者：
Cheng, LL;Mudge, AW
人们普遍认为，哺乳动物许旺细胞不表达P-0，即外周髓鞘中的主要糖蛋白，除非它们被轴突信号诱导这样做，轴突信号可以被触发cAMP信号传导途径的试剂模拟。相比之下，我们发现培养的雪旺细胞产生大量的P-0而不添加任何轴突样信号，只要它们在培养过程中没有暴露于血清。我们还报告，胶质细胞生长因子/神经调节蛋白抑制这种组成性P-0的表达。髓鞘碱性蛋白以类似的方式调节。我们认为，表达的P-0的许旺细胞髓鞘形成的发病前可能会被阻止的神经内的抑制信号，而不是由轴突的阳性信号的情况下。
It is widely thought that mammalian Schwann cells do not express P-0, the major glycoprotein in peripheral myelin, unless they are induced to do so by axonal signals that can be mimicked by agents that trigger cAMP signaling pathways. In contrast, we find that cultured Schwann cells make large amounts of P-0 without the addition of any axonal-like signal, provided they have not been exposed to serum during the culture process. We also report that glial growth factor/neuregulin inhibits this constitutive P-0 expression. Myelin basic protein is regulated in a similar way. We suggest that expression of P-0 by Schwann cells before the onset of myelination may be prevented by inhibitory signals within the nerve, rather than by the absence of a positive signal from axons.