RESPONSE-SURFACE MODEL FOR PREDICTING THE EFFECTS OF TEMPERATURE PH, SODIUM-CHLORIDE CONTENT, SODIUM-NITRITE CONCENTRATION AND ATMOSPHERE ON THE GROWTH OF LISTERIA-MONOCYTOGENES
RESPONSE-SURFACE MODEL FOR PREDICTING THE EFFECTS OF TEMPERATURE PH, SODIUM-CHLORIDE CONTENT, SODIUM-NITRITE CONCENTRATION AND ATMOSPHERE ON THE GROWTH OF LISTERIA-MONOCYTOGENES
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DOI：
10.4315/0362-028x-53.5.370
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发表时间：
1990-05-01
影响因子：
2
通讯作者：
PHILLIPS, JG
中科院分区：
文献类型：
--
作者：
BUCHANAN, RL;PHILLIPS, JG
采用析因加补充中心复合实验设计，定量评价了温度(5℃~37℃)、pH值(4.5~7.5)、氯化钠(5~45g/L)、亚硝酸钠(0~1000u.g/ml)和气氛(好氧与厌氧)对单核细胞增多性李斯特氏菌在磷酸胰酶发酵液中生长动力学的影响及其交互作用。总共生成了709条生长曲线，其中个别曲线使用非线性回归分析和Gompertz函数进行了拟合。用响应面分析的响应面对结果进行分析，生成一个三次模型来预测单核细胞增多性李斯特菌在指定范围内变量的任意组合的生长。对该模型的评价表明，该模型可以对食品配方和储存条件对单核细胞增多性李斯特菌生长的影响提供合理的“初步估计”，并且可以很容易地自动开发出“用户友好的”软件。
A factorial plus supplemental central composite experimental design was used to assess quantitatively the effects and interactions of temperature (5.degree.-37.degree.C), pH (4.5-7.5), NaCl (5-45 g/l), NaNO2 (0-1000 .mu.g/ml), and atmosphere (aerobic vs. anaerobic) on the growth kinetics of Listeria monocytogenes Scott A in Tryptose Phosphate Broth. A total of 709 growth curves were generated, with individual curves fitted using non-linear regression analysis in conjunction with the Gompertz function. The results were analyzed by response surface analyzed by response surface analysis to generate a cubic model to predict the growth of L. monocytogenes in response to any combination of the the variables within the specified ranges. Evaluation of the model indicated that it can be used to provide reasonable "first estimates" of the impact of food formulation and storage conditions on the growth of L. monocytogenes, and can be automated readily to develop "user-friendly" software.