In vitro and in vivo toxicity of CdTe nanoparticles.
In vitro and in vivo toxicity of CdTe nanoparticles.
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DOI：
10.1166/jnn.2007.125
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发表时间：
2007-02
影响因子：
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通讯作者：
Yongbin Zhang;Wei Chen;Jun Zhang;Jing Liu;Guangping Chen;C. Pope
中科院分区：
文献类型：
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作者：
Yongbin Zhang;Wei Chen;Jun Zhang;Jing Liu;Guangping Chen;C. Pope
碲化镉纳米颗粒具有强而稳定的荧光，在生物探测、固态照明等领域具有广阔的应用前景。纳米颗粒的毒性评价对于实现这些实际应用具有重要意义。然而，关于镉镉纳米颗粒毒性的系统研究尚未见报道。我们采用四甲基偶氮唑盐比色法研究和比较了纳米镉镉对人肝癌细胞株HepG2的细胞毒作用及其大小和浓度依赖关系。CdTe纳米颗粒以浓度和大小依赖的方式引起细胞毒性，较小的颗粒表现出更高的效力。部分纯化的CdTe-Red颗粒(甲醇沉淀和再悬浮后)的细胞毒性较小，表明游离镉离子可能参与了细胞毒性。我们还评估了镉镉红颗粒静脉染毒(2微摩尔/公斤)对雄性大鼠的急性毒性。几乎没有注意到功能性毒性或临床(尿液或血液)变化的迹象。有趣的是，运动活动一过性降低(治疗后2小时)，然后在稍后的时间点显著增加(服药后24小时)。这些研究为进一步表征不同类型的CdTe纳米颗粒的体外和体内毒性潜力提供了一个框架，并表明在某些条件下这些纳米颗粒可能是神经系统的靶点。
Cadmium telluride (CdTe) nanoparticles exhibit strong and stable fluorescence that is attractive for many applications such as biological probing and solid state lighting. The evaluation of nanoparticle toxicity is important for realizing these practical applications. However, no systematic studies of CdTe nanoparticle toxicity have been reported. We investigated and compared the size- and concentration-dependent cytotoxicity of CdTe nanoparticles in human hepatoma HepG2 cells using the MTT assay. CdTe nanoparticles elicited cytotoxicity in a concentration- and size-dependent manner, with smaller-sized particles exhibiting somewhat higher potency. Lesser cytotoxicity of partially purified CdTe-Red particles (following methanol precipitation and resuspension) suggested that free cadmium ions may contribute to cytotoxicity. We also evaluated the acute toxicity of CdTe-Red particles following intravenous exposure in male rats (2 micromol/kg). Few signs of functional toxicity or clinical (urinary or blood) changes were noted. Interestingly, motor activity was transiently reduced (2 hours after treatment) and then significantly increased at a later timepoint (24 hours after dosing). These studies provide a framework for further characterizing the in vitro and in vivo toxic potential of different types of CdTe nanoparticles and suggest that the nervous system may be targeted by these nanoparticles under some conditions.