Comparative immunogenicity analysis of modified vaccinia Ankara vectors expressing native or modified forms of hepatitis C virus E1 and E2 glycoproteins
Comparative immunogenicity analysis of modified vaccinia Ankara vectors expressing native or modified forms of hepatitis C virus E1 and E2 glycoproteins
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DOI：
10.1016/j.vaccine.2004.04.005
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发表时间：
2004-09-28
期刊：
影响因子：
5.5
通讯作者：
Kieny, MP
中科院分区：
文献类型：
--
作者：
Abraham, JD;Himoudi, N;Kieny, MP
我们已经在C57[1316]和HLA-A2.1转基因小鼠中评估了表达天然HCV E1 E2多蛋白的三种MVA载体的免疫原性。截短的和分泌的E1（E '1（311））和E2（E' 2（661））蛋白，或呈递在质膜上的嵌合E1 E2异二聚体。HLA-A2.1转基因小鼠免疫后主要诱导Th 1型免疫应答，而C57/B16小鼠免疫后主要诱导Th 2型免疫应答。三种载体的比较显示当抗原被分泌或膜结合时体液应答增加，并且当抗原暴露在细胞表面上时细胞应答轻微增加。(C)2004爱思唯尔有限公司保留所有权利。
We have evaluated in C57[1316 and HLA-A2.1 transgenic mice the immunogenicity of three MVA vectors expressing either native HCV El E2 polyprotein. truncated and secreted El (E'1 (311)) and E2 (E'2(661)) proteins, or a chimeric El E2 heterodimer presented at the plasma membrane. Immunization induced mainly a Th1 response in HLA-A2.1 transgenic mice while a Th2-type response was detected in C57/B16 mice. Comparison of the three vectors shows an increase in the humoral response when antigens are secreted or membrane bound, and slightly in the cellular response when antigens are exposed on the cell surface. (C) 2004 Elsevier Ltd. All rights reserved.