Analysis of a baculovirus lacking a functional viral fibroblast growth factor homolog
Analysis of a baculovirus lacking a functional viral fibroblast growth factor homolog
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DOI：
10.1016/j.virol.2006.01.016
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发表时间：
2006-03-15
期刊：
影响因子：
3.7
通讯作者：
Passarelli, AL
中科院分区：
文献类型：
--
作者：
Detvisitsakun, C;Hutfless, EL;Passarelli, AL
杆状病毒编码成纤维细胞生长因子（vfgf）同源物，其在病毒感染期间的功能尚不清楚。我们构建了缺乏功能性 vfgf 的苜蓿银纹夜蛾 M 核多角体病毒 (AcMNPV) 的重组杆粒，并在两种昆虫细胞系中对其进行了表征。在高复数和低复数感染下，两种细胞系中亲本病毒和 vfgf 缺陷病毒中出芽病毒产生的动力学相似。此外，突变体病毒和亲本病毒在蛋白质或DNA合成方面没有观察到明显差异。最后，含有 vfgf 和缺乏 vfgf 的病毒的共感染以及传代几代并没有显示出这两种病毒具有一致的生长优势。 (C) 2006 Elsevier Inc. 保留所有权利。
Baculoviruses encode fibroblast growth factor (vfgf) homologs whose function during vir-us infection is unknown. We constructed a recombinant bacmid of Autographa californica M nucleopolyhedrovirus (AcMNPV) lacking a functional vfgf and characterized it in two insect cell lines. The kinetics of budded virus production were similar in the parental and vfgf-deficient viruses in both cell lines at both high and tow multiplicities of infection. In addition, no obvious differences were observed between the mutant and parental viruses in protein or DNA synthesis. Finally, coinfection of vfgf-containing and -deficient viruses and passage for several generations did not reveal a consistent growth advantage for either virus. (C) 2006 Elsevier Inc. All rights reserved.