High oxygen atmosphere for neuronal cell culture with nerve growth factor. II. Survival and growth of clonal rat pheochromocytoma PC12h cells
High oxygen atmosphere for neuronal cell culture with nerve growth factor. II. Survival and growth of clonal rat pheochromocytoma PC12h cells
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用于含有神经生长因子的神经元细胞培养的高氧气氛。
DOI：
10.1016/0006-8993(90)90004-u
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
H. Hatanaka
中科院分区：
文献类型：
--
作者：
Y. Enokido;H. Hatanaka
克隆性大鼠嗜铬细胞瘤PC12h细胞在50%O2气氛中培养时，细胞逐渐死亡。而加入终浓度为50 ng/ml的NGF则可使细胞免于死亡。在40%O2气氛中培养的PC12h细胞，与正常的20%O2气氛中培养的PC12h细胞相比，对细胞生长的影响不大。很高的O2浓度(60%)对PC12h细胞的生长有严重的损伤作用，NGF对细胞生长的恢复似乎是不够的。即使在50%O2气氛中，NGF也能使PC12h细胞充分分化并延伸出致密的长突起。然而，在60%O2气氛中培养的神经突起伸展受到抑制。在50%O2条件下，NGF对细胞死亡的挽救作用呈剂量依赖性，其ED50值为5 ng/ml，碱性成纤维细胞生长因子和表皮生长因子对高O2培养的细胞死亡也有明显的挽救作用，而胰岛素则无此作用。由于NGF对PC12h细胞的分化作用被认为为研究NGF对神经元的影响提供了一个模型系统，因此，本研究结果提示，NGF诱导分化的PC12h细胞可能存在一种对神经元的高氧毒性保护机制，而未分化的PC12h细胞则不存在这种机制。
When clonal rat pheochromocytoma PC12h cells were cultured in a 50% O2atmosphere, cells gradually died during the cultivation. On the other hand, the addition of NGF at the final concentration of 50 ng/ml could rescue the cells from death. The culture in a 40% O2atmosphere had little effect on the growth of PC12h cells, as compared with the culture in a normal 20% O2condition. A very high O2concentration, as 60%, caused severe damage to PC12h cell growth, and the restoration of cell growth by NGF seemed to be insufficient. PC12h cells were fully differentiated and extended dense long neurites by NGF even in a 50% O2atmosphere. However, the neurite extension in the culture in a 60% O2atmosphere was suppressed. The cell-saving effect of NGF on cell death in culture under a 50% O2atmosphere was dose-dependent, and theED50value of NGF was 5 ng/ml. Basic fibroblast growth factor and epidermal growth factor also had a potent effect to rescue the cell death in the high O2culture, but insulin had no effect. Since the differentiation effects of NGF on PC12h cells are thought to offer a model system to investigate the effect of NGF on neurons, the present observations suggest that a protection machinery for high O2toxicity to neurons may exist in the neuronal differentiated PC12h cells by NGF, but not in the undifferentiated cells.
新型神经营养因子、受体和癌基因。
影响因子：
13.9
作者：
Walicke,PA
通讯作者：
Walicke,PA