Cell movements during epiboly and gastrulation in zebrafish.
Cell movements during epiboly and gastrulation in zebrafish.
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DOI：
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发表时间：
1990-04
期刊：
影响因子：
4.6
通讯作者：
R. M. Warga;C. Kimmel
中科院分区：
文献类型：
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作者：
R. M. Warga;C. Kimmel
从斑马鱼的囊胚晚期开始，位于胚盘表面上皮层下的细胞经历重排，伴随着胚胎形状的重大变化。我们描述了三种不同类型的细胞重排。(1)外生期的放射状细胞嵌入胚盘中，混合细胞位于胚盘较浅的部位。随着胚盘变薄并遍布卵黄细胞，这些重排彻底搅动了深层细胞的位置。(2)在胚盘边缘或其附近的内卷化发生在原肠胚形成期间。这种运动将胚盘折叠成两个细胞层，上胚层和下胚层，在围绕其整个圆周的环（胚环）内。内卷细胞在下胚层中相对于留在上胚层中的细胞向前移动;这种移动剪切了原肠胚形成前相邻细胞的位置。退化细胞最终形成内胚层和中胚层，根据退化时间的前后顺序。外胚层相当于胚胎外胚层。(3)中外侧细胞嵌入在上胚层和下胚层介导的收敛和扩展运动向背侧的原肠胚。通过这种重排，最初彼此相邻的细胞沿着胚胎的前后轴沿着分散。斑马鱼的外突、内卷和会聚延伸涉及与两栖动物相同的细胞重排，并且它们发生在胚胎发生的可比阶段。
Beginning during the late blastula stage in zebrafish, cells located beneath a surface epithelial layer of the blastoderm undergo rearrangements that accompany major changes in shape of the embryo. We describe three distinctive kinds of cell rearrangements. (1) Radial cell intercalations during epiboly mix cells located deeply in the blastoderm among more superficial ones. These rearrangements thoroughly stir the positions of deep cells, as the blastoderm thins and spreads across the yolk cell. (2) Involution at or near the blastoderm margin occurs during gastrulation. This movement folds the blastoderm into two cellular layers, the epiblast and hypoblast, within a ring (the germ ring) around its entire circumference. Involuting cells move anteriorwards in the hypoblast relative to cells that remain in the epiblast; the movement shears the positions of cells that were neighbors before gastrulation. Involuting cells eventually form endoderm and mesoderm, in an anterior-posterior sequence according to the time of involution. The epiblast is equivalent to embryonic ectoderm. (3) Mediolateral cell intercalations in both the epiblast and hypoblast mediate convergence and extension movements towards the dorsal side of the gastrula. By this rearrangement, cells that were initially neighboring one another become dispersed along the anterior-posterior axis of the embryo. Epiboly, involution and convergent extension in zebrafish involve the same kinds of cellular rearrangements as in amphibians, and they occur during comparable stages of embryogenesis.