Long non-coding RNA and MicroRNA profiling provides comprehensive insight into non-coding RNA involved host immune responses in ALV-J-infected chicken primary macrophage
Long non-coding RNA and MicroRNA profiling provides comprehensive insight into non-coding RNA involved host immune responses in ALV-J-infected chicken primary macrophage
复制标题
长非编码 RNA 和 MicroRNA 分析可全面了解 ALV-J 感染鸡原代巨噬细胞中涉及宿主免疫反应的非编码 RNA
DOI：
10.1016/j.dci.2019.103414
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发表时间：
2019-11-01
影响因子：
2.9
通讯作者：
Zhang, Xiquan
中科院分区：
文献类型：
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作者：
Dai, Manman;Feng, Min;Zhang, Xiquan
禽白血病病毒亚群J （ALV-J）感染可引起鸡的肿瘤和免疫抑制。巨噬细胞在宿主防御入侵病原体中起着至关重要的作用。本研究采用全转录组分析方法，分析鸡原代单核细胞源性巨噬细胞（MDMs）的宿主因子，包括基因、microRNA （miRNA）、长链非编码RNA （lncRNA）及其调控网络。在ALV-J感染后3小时，在MDMs中共鉴定出128个差异表达（DE） lncrna和15个DE mirna，在ALV-J感染后36小时，在MDMs中鉴定出30个DE lncrna和8个DE mirna。我们进一步构建了DE lncrnas - mrna、mirna - mrna和lncrnas - mirna - mrna相互作用网络。结果表明，在alv - j感染的MDMs中，3 hpi时DE lncRNAs和miRNAs通过ceRNA网络参与Jak-STAT信号通路CCND3和SOCS5的调控。此外，包括XLOC_672329、ALDBGALG0000001429、XLOC_016500和ALDBGALG0000000253在内的lncrna在3 hpi时分别顺式调节MDMs中的CH25H、CISH、IL-1 β和CD80参与宿主抗病毒应答。本研究结果全面揭示了鸡原代巨噬细胞中非编码RNA与ALV-J之间的联系，为进一步研究表观遗传对ALV-J抗病育种的影响以及免疫系统和基因组研究提供了良好的资源。
Avian leukosis virus subgroup J (ALV-J) infection can cause tumors and immunosuppression in infected chickens. Macrophages play a crucial role in host defense against invading pathogens. In the present study, whole transcriptome analysis was performed to analyze the host factors including genes, microRNA (miRNA), long non-coding RNA (lncRNA) and their regulatory network in chicken primary monocyte-derived macrophages (MDMs). In total, 128 differentially expressed (DE) lncRNAs and 15 DE miRNAs were identified in MDMs at 3 h post infection (hpi), and 30 DE lncRNAs and 8 DE miRNAs were identified in MDMs at 36 hpi during ALV-J infection. We further constructed the DE lncRNAs-mRNAs, miRNA-mRNAs and lncRNAs-miRNA-mRNAs interaction networks. The results suggested that DE lncRNAs and miRNAs are involved in the regulation of CCND3 and SOCS5 in Jak-STAT signaling pathway via ceRNA network in ALV-J-infected MDMs at 3 hpi. In addition, lncRNAs including XLOC_672329, ALDBGALG0000001429, XLOC_016500 and ALDBGALG0000000253 cis-regulating CH25H, CISH, IL-1 beta and CD80 respectively in MDMs at 3 hpi participated in host antiviral responses. Our findings give a comprehensive view of the connection between non-coding RNA and ALV-J in chicken primary macrophages, and provide an excellent resource for further studies of epigenetic effects on ALV-J disease resistance breeding as well as immune system and genomic researches.