An olfactory neuron responds stochastically to temperature and modulates Caenorhabditis elegans thermotactic behavior
An olfactory neuron responds stochastically to temperature and modulates Caenorhabditis elegans thermotactic behavior
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DOI：
10.1073/pnas.0805004105
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发表时间：
2008-08-05
影响因子：
11.1
通讯作者：
Sengupta, Piali
中科院分区：
文献类型：
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作者：
Biron, David;Wasserman, Sara;Sengupta, Piali
秀丽隐杆线虫通过使用一种由其培养温度（T-c）记忆调节的行为策略来导航温度梯度。在高于或接近摄氏零度的温度下，动物通过调节随机重新定向事件的速率来对温度变化做出反应。双侧AFD神经元被认为是热感觉神经元，但其他的热感觉神经元也被预测在调节热致行为中发挥作用。在这里，我们展示了AWC嗅觉神经元对温度的反应。与AFD神经元以连续的、分级的钙信号响应热刺激不同，AWC神经元表现出随机的钙事件，其频率以t -c依赖的方式与刺激相关。缺乏AWC神经元或AWC神经元过度活跃的动物在热致行为的重定向率调节上存在缺陷。我们的观察表明，AFD和AWC神经元通过不同的策略编码热刺激来调节线虫的热致行为。
Caenorhabditis elegans navigates thermal gradients by using a behavioral strategy that is regulated by a memory of its cultivation temperature (T-c). At temperatures above or around the T-c, animals respond to temperature changes by modulating the rate of stochastic reorientation events. The bilateral AFD neurons have been implicated as thermosensory neurons, but additional thermosensory neurons are also predicted to play a role in regulating thermotactic behaviors. Here, we show that the AWC olfactory neurons respond to temperature. Unlike AFD neurons, which respond to thermal stimuli with continuous, graded calcium signals, AWC neurons exhibit stochastic calcium events whose frequency is stimulus-correlated in a T-c-dependent manner. Animals lacking the AWC neurons or with hyperactive AWC neurons exhibit defects in the regulation of reorientation rate in thermotactic behavior. Our observations suggest that the AFD and AWC neurons encode thermal stimuli via distinct strategies to regulate C elegans thermotactic behavior.