Flavobacterium ranwuense sp. nov., isolated from glacier
Flavobacterium ranwuense sp. nov., isolated from glacier
复制标题
然乌黄杆菌 sp.
DOI：
10.1099/ijsem.0.003687
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发表时间：
2019
影响因子：
2.8
通讯作者：
Xin Yuhua
中科院分区：
文献类型：
--
作者：
hang Guoqing;Liu Qing;Liu Hongcan;Zhou Yuguang;Xin Yuhua
16S rRNA基因序列分析结果表明，菌株lb2p22t属于黄杆菌属，与laiguenseLB2P30T（98.14%）、glacieiCGMCC 1.5380T（98.02%）、psychroterraeCCM 8827T（97.94%）和granuliCGMCC 1.10125T（97.91%）相似度最高。菌株lb2p22与近缘种的平均核苷酸同源性为77.99 ~ 84.99%，数字DNA-DNA杂交值为22.3 ~ 29.3%，为黄杆菌属新种。菌株lb2p22t在0-25℃、pH 7.0-8.0和高达1.0% (w/v) NaCl条件下生长。主要的细胞脂肪酸是iso-C15: 0，总结特征3（包括C16: 1ω7c/C16: 1ω6c）和前iso-C15: 0。基因组DNA G+C含量为34.3% mol%。甲基萘醌主要为MK-6，极性脂质为磷脂酰乙醇胺、1种未识别的氨基脂质和4种未识别的脂质。基于这些数据，一个新的物种，黄杆菌。采用LB2P22T（=CGMCC 1.11361T=NBRC 113777T）作为型应变。
A Gram-stain-negative, aerobic, rod-shaped, devoid of flagella, orange–yellow-coloured bacterium (strain LB2P22T) was isolated from an ice sample collected from the Laigu glacier on the Tibetan Plateau, PR China. The results of 16S rRNA gene sequence analysis indicated that strain LB2P22Tbelongs to the genusFlavobacteriumwith highest similarity toFlavobacterium laiguenseLB2P30T(98.14 %),Flavobacterium glacieiCGMCC 1.5380T(98.02 %),Flavobacterium psychroterraeCCM 8827T(97.94 %) andFlavobacterium granuliCGMCC 1.10125T(97.91 %). Strain LB2P22Thad 77.99–84.99 % average nucleotide identity and 22.3–29.3 % digital DNA–DNA hybridization values with its closest relatives, indicating that it represents a novel species of the genusFlavobacterium. Strain LB2P22Tgrew at 0–25 °C, pH 7.0–8.0 and up to 1.0 % (w/v) NaCl. The main cellular fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c/C16 : 1ω6c) and anteiso-C15 : 0. The genomic DNA G+C content is 34.3 mol%. The predominant menaquinone was MK-6 and the polar lipids were phosphatidylethanolamine, one unidentified aminolipid and four unidentified lipids. Based on these data, a novel species,Flavobacterium ranwuensesp. nov., is proposed, with LB2P22T(=CGMCC 1.11361T=NBRC 113777T) as the type strain.