Looking and listening to light: the evolution of whole-body photonic imaging
Looking and listening to light: the evolution of whole-body photonic imaging
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DOI：
10.1038/nbt1074
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发表时间：
2005-03-01
影响因子：
46.9
通讯作者：
Weissleder, R
中科院分区：
文献类型：
--
作者：
Ntziachristos, V;Ripoll, J;Weissleder, R
活体动物的光学成像已经发展成为生物医学研究中的重要工具，因为光子技术和报告策略的进步已经导致对体内生物过程的广泛探索。虽然显微镜已经得到了很大的关注，宏观成像已经允许小动物成像具有更大的视野（从几毫米到几厘米，取决于实施）。摄影方法一直是整个动物荧光和生物发光宏观检查的支柱，但重点正在转向光子方法，该方法使用断层摄影原理，以高灵敏度和亚毫米到毫米分辨率在几毫米到厘米的深度非侵入性成像光学对比度。最近的理论和仪器的进步允许使用大数据集和多个投影，并提供定量，三维全身图像的实用系统。然而，光子成像要充分发挥其潜力，还需要在改进光学反演方法和数据采集技术方面取得进一步进展。
Optical imaging of live animals has grown into an important tool in biomedical research as advances in photonic technology and reporter strategies have led to widespread exploration of biological processes in vivo. Although much attention has been paid to microscopy, macroscopic imaging has allowed small-animal imaging with larger fields of view (from several millimeters to several centimeters depending on implementation). Photographic methods have been the mainstay for fluorescence and bioluminescence macroscopy in whole animals, but emphasis is shifting to photonic methods that use tomographic principles to noninvasively image optical contrast at depths of several millimeters to centimeters with high sensitivity and sub-millimeter to millimeter resolution. Recent theoretical and instrumentation advances allow the use of large data sets and multiple projections and offer practical systems for quantitative, three-dimensional whole-body images. For photonic imaging to fully realize its potential, however, further progress will be needed in refining optical inversion methods and data acquisition techniques.