Mesenchymal Stem Cell Sheets Exert Antistenotic Effects in a Rat Arterial Injury Model
Mesenchymal Stem Cell Sheets Exert Antistenotic Effects in a Rat Arterial Injury Model
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DOI：
10.1089/ten.tea.2018.0030
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发表时间：
2018-07-23
影响因子：
4.1
通讯作者：
Shimizu, Tatsuya
中科院分区：
文献类型：
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作者：
Homma, Jun;Sekine, Hidekazu;Shimizu, Tatsuya
导管或手术干预后的呼吸实质上影响动脉闭塞性疾病的预后。间充质干细胞（MSC）可能对损伤的动脉具有抗狭窄作用。从动脉外膜侧移植MSC比血管内移植更安全，但尚未得到广泛的研究。本研究采用大鼠股动脉损伤模型，比较移植细胞片和移植细胞悬液的抗狭窄作用。大鼠脂肪源性干细胞（ASC）被用作MSC的来源。对于细胞片和悬浮液，在动脉损伤当天移植6 × 106个MSC。MSC片比MSC悬浮液更能减弱新生内膜增生（苏木精/伊红染色切片中内膜-中膜比率：0.55 +/- 0.13 vs. 1.14 +/- 0.12; p
Restenosis after catheter or surgical intervention substantially affects the prognosis of arterial occlusive disease. Mesenchymal stem cells (MSCs) may have antistenotic effects on injured arteries. MSC transplantation from the adventitial side of an artery is safer than endovascular transplantation but has not been extensively examined. In this study, a rat model of femoral artery injury was used to compare the antistenotic effects of transplanted cell sheets and transplanted cell suspensions. Rat adipose-derived stem cells (ASCs) were used as the source of MSCs. For both cell sheets and suspensions, 6x10(6) MSCs were transplanted on the day of arterial injury. MSC sheets attenuated neointimal hyperplasia more than MSC suspensions (intima-to-media ratio in hematoxylin/eosin-stained sections: 0.55 +/- 0.13 vs. 1.14 +/- 0.12; p