Reduction of variation in T-cell subset enumeration among 55 laboratories using single-platform, three or four-color flow cytometry based on CD45 and SSC-based gating of lymphocytes
Reduction of variation in T-cell subset enumeration among 55 laboratories using single-platform, three or four-color flow cytometry based on CD45 and SSC-based gating of lymphocytes
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DOI：
10.1002/cyto.10084
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发表时间：
2002-04-15
期刊：
影响因子：
--
通讯作者：
Barnett, D
中科院分区：
文献类型：
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作者：
Gratama, JW;Kraan, J;Barnett, D
背景：CD4（+）和CD8（+）T细胞子集的枚举提供了相关信息，用于诊断和监测细胞免疫缺陷患者。结果，1995年在比利时，荷兰和卢森堡实施了一项外部质量保证计划。举办了一个研讨会，以培训参与者的最先进技术，以评估绝对T细胞子集（即，即，即，即基于CD45和侧向光散射的三个或四色单平面测定，其目的是实现位置间系数变异（CVS）
Background: Enumeration of CD4(+) and CD8(+) T-cell subsets provides relevant information for diagnosis and monitoring of patients with cellular immunodeficiencies. As a result, an external quality assurance scheme was implemented in Belgium, The Netherlands, and Luxemburg in 1995. A workshop was held to train the participants in state-of-the art technology for assessment of absolute T-cell subset counts (i.e., a three or four-color, single-platform assay with lymphocyte gating based on CD45 and sideward light scatter) with the aim to achieve between-site coefficients of variation (CVs)