Gain of 3q26: A genetic marker in low-grade squamous intraepithelial lesions (LSIL) of the uterine cervix
Gain of 3q26: A genetic marker in low-grade squamous intraepithelial lesions (LSIL) of the uterine cervix
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DOI：
10.1016/j.ygyno.2009.03.031
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发表时间：
2009-07-01
影响因子：
4.7
通讯作者：
Kilpatrick, Michael W.
中科院分区：
文献类型：
--
作者：
Seppo, Antti;Jalali, G. Reza;Kilpatrick, Michael W.
Objective.医生几乎没有资源来确定哪些LSIL将进展为HSIL或退化。最近发现染色体3q 26区域在宫颈癌患者中扩增。这种3q增益的频率随着发育不良的严重程度而增加。本研究的主要目的是评估用于检测液体细胞学样本中3q增益的自动FISH测定作为风险分层和分诊的潜在工具。从257份液体细胞学标本制备的载玻片（97份阴性。135 LSIL 25 HSIL）与染色体3q 26区域的单拷贝探针和染色体7的着丝粒α-重复序列的探针杂交，使用标准FISH方法。使用自动化分析，使用20倍物镜确定3q 26的细胞核总数和具有> 2个信号的细胞核数目。基于3q 26 FISH信号的数量对细胞核进行等级排序。800个细胞核与最高数量的信号进行了评分，使用FISH探针和细胞核与增加数量的3q信号进行了枚举。对257例标本的分析表明，全自动FISH评分系统可以检测液体细胞学样本中的3q增益。用于测定液体细胞学中3q增益的全自动方法可能是实施常规检测所必需的测定。需要进行更多的研究来验证这项技术的实用性。(C)2009 Elsevier Inc. All rights reserved.
Objective. Physicians have few resources for determining which LSIL will progress to HSIL or regress. Recently the chromosome 3q26 region was found to be amplified in patients with cervical cancer. The frequency of this 3q gain increased with severity of dysplasia. The primary objective of this study was to evaluate an automated FISH assay for detection of 3q gain in liquid cytology samples as a potential tool for risk stratification and triaging.Methods. Slides prepared from 257 liquid cytology specimens (97 Negative. 135 LSIL 25 HSIL) were hybridized with a single-copy probe for the chromosome 3q26 region and a probe for the centromeric alpha-repeat sequence of chromosome 7, using standard FISH methods. Using automated analysis, the total number of nuclei and the number of nuclei with > 2 signals for 3q26 were determined, using a 20x objective. The nuclei were rank ordered based on number of 3q26 FISH signals. The 800 nuclei with the highest number of signals were scored using both FISH probes and nuclei with increased numbers of 3q signals were enumerated.Results and conclusions. Analysis of 257 specimens demonstrated that a fully automated FISH scoring system can detect 3q gain in liquid cytology samples. A fully automated method for determination of 3q gain in liquid cytology may be the assay necessary to implement routine testing. Additional studies to validate the utility of this technology are needed. (C) 2009 Elsevier Inc. All rights reserved.