Listeria monocytogenes develops no resistance to ferulic acid after exposure to low concentrations
Listeria monocytogenes develops no resistance to ferulic acid after exposure to low concentrations
复制标题
单核细胞增生李斯特菌在暴露于低浓度阿魏酸后不会产生耐药性
DOI：
10.1016/j.foodcont.2014.07.062
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发表时间：
2014
期刊：
影响因子：
6
通讯作者：
Bon Kimura
中科院分区：
文献类型：
--
作者：
Hajime Takahashi;Kaoru Takada;Tomoki Tsuchiya;Satoko Miya;Takashi Kuda;Bon Kimura
抗微生物剂已被用作控制单核细胞增生李斯特菌生长的添加剂。虽然乳酸链球菌素已被用于抑制食品中单核细胞增生李斯特菌的生长，但暴露于这种制剂也会导致乳酸链球菌素耐药性的发展。阿魏酸也被广泛用于抑制单核细胞增生李斯特菌的生长，尽管以前没有对阿魏酸耐药性的发展进行过研究。在本研究中，我们将含有这两种抗菌剂之一的培养基与先前暴露于这些药物的细菌接种，并评估耐药性的任何变化。5 ppm Nisin处理后，单核细胞增生李斯特菌对Nisin的抗性增强，对20 ppm Nisin的存活率提高。另一方面，暴露于1000 ppm阿魏酸不会增加阿魏酸抗性。本研究表明，必须仔细考虑添加乳链菌肽的浓度以达到足够的效果。另一方面，阿魏酸显示出产生耐药性细菌的风险最小，从而使其具有稳定的效果。
Antimicrobial agents have been used as additives to control the growth of Listeria monocytogenes. Although nisin has been used to inhibit the growth ofL. monocytogenesin food products, exposure to this agent also poses a risk for the development of nisin resistance. Ferulic acid has also been extensively used for growth inhibition ofL. monocytogenes, although no previous investigations on the development of ferulic acid resistance have been conducted. In the present study, we inoculated culture media containing one of these two antimicrobial agents with bacteria that had been previously exposed to these agents and assessed for any changes in resistance. The resistance ofL. monocytogenesto nisin increased after exposure to 5 ppm nisin, and its survival rate against 20 ppm nisin improved. On the other hand, exposure to 1000 ppm ferulic acid did not increase ferulic acid resistance. The present study demonstrated that the concentration of added nisin must be considered carefully to achieve a sufficient effect. Ferulic acid, on the other hand, showed a minimal risk of producing resistant bacteria, thus allowing it to impart a stable effect.