Nef enhances c-Cbl phosphorylation in HIV-infected CD4+ T lymphocytes.
Nef enhances c-Cbl phosphorylation in HIV-infected CD4+ T lymphocytes.
复制标题
Nef 增强 HIV 感染的 CD4 T 淋巴细胞中的 c-Cbl 磷酸化。
DOI：
10.1016/j.virol.2005.03.021
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发表时间：
2005
期刊：
影响因子：
3.7
通讯作者：
Henderson,AndrewJ
中科院分区：
文献类型：
--
作者：
Yang,Polung;Henderson,AndrewJ
The multifunctional HIV-1 protein Nef possesses several motifs that interact with signaling molecules in infected T cells. In order to determine whether Nef influences T cell activation, cells were infected with Nef-positive and Nef-negative clones of HIV. CD28 expression and changes in tyrosine phosphorylation were monitored. We observed no Nef-dependent changes in CD28 expression or function. However, infection with Nef-positive virus led to changes in tyrosine phosphorylation. This Nef-induced phosphorylation was observed in unstimulated cells, and c-Cbl was identified as one of the proteins whose phosphorylation was upregulated by Nef. Furthermore, Lck is required for Nef-mediated c-Cbl tyrosine phosphorylation. These results suggest that Nef modifies T cell signaling in the absence of T cell receptor engagement and co-stimulation.
