Efficient production and secretion of pyruvate from Halomonas sp. KM-1 under aerobic conditions.
Efficient production and secretion of pyruvate from Halomonas sp. KM-1 under aerobic conditions.
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DOI：
10.1186/s13568-016-0195-y
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发表时间：
2016-03
期刊：
影响因子：
3.7
通讯作者：
Tsubota J
中科院分区：
文献类型：
--
作者：
Kawata Y;Nishimura T;Matsushita I;Tsubota J
嗜碱、嗜盐细菌Halomonassp. KM-1可利用己糖和戊糖在好氧条件下进行生物塑料聚（R）-3-羟基丁酸（PHB）的细胞内储存。在这项研究中，我们研究了硝酸钠浓度的影响，对聚羟基丁酸在KM-1菌株的积累。出乎意料的是，我们观察到丙酮酸的分泌，丙酮酸是所有生物体中碳和能量代谢过程的中心中间体;因此，丙酮酸被广泛用作药物工业生物合成的起始材料，并用于生产作物保护剂，聚合物，化妆品和食品添加剂。然后，我们进一步分析了培养温度和缓冲液浓度变化后的丙酮酸生产率。在48 h的分批培养实验中，野生型盐单胞菌KM-1的丙酮酸产量为63.3 g/L，丙酮酸的分泌速率为1.32 g/（L·h），与突变菌和重组菌的结果相当。因此，这些数据提供了重要的见解丙酮酸生产使用这种新的菌株。
The alkaliphilic, halophilic bacterium Halomonas sp. KM-1 can utilize both hexose and pentose sugars for the intracellular storage of bioplastic poly-(R)-3-hydroxybutyric acid (PHB) under aerobic conditions. In this study, we investigated the effects of the sodium nitrate concentration on PHB accumulation in the KM-1 strain. Unexpectedly, we observed the secretion of pyruvate, a central intermediate in carbon- and energy-metabolism processes in all organisms; therefore, pyruvate is widely used as a starting material in the industrial biosynthesis of pharmaceuticals and is employed for the production of crop-protection agents, polymers, cosmetics, and food additives. We then further analyzed pyruvate productivity following changes in culture temperature and the buffer concentration. In 48-h batch-cultivation experiments, we found that wild-type Halomonas sp. KM-1 secreted 63.3 g/L pyruvate at a rate of 1.32 g/(L·h), comparable to the results of former studies using mutant and recombinant microorganisms. Thus, these data provided important insights into the production of pyruvate using this novel strain.