BATTLE: Genetically Engineered Strategies for Split-Tunable Allocation of Multiple Transgenes in the Nervous System
BATTLE: Genetically Engineered Strategies for Split-Tunable Allocation of Multiple Transgenes in the Nervous System
复制标题
DOI：
10.1016/j.isci.2020.101248
复制
发表时间：
2020-06-26
期刊：
影响因子：
5.8
通讯作者：
Kawashima, Chiho
中科院分区：
文献类型：
--
作者：
Kohara, Keigo;Inoue, Akitoshi;Kawashima, Chiho
阐明细胞和突触连接的精细结构和功能需要开发新的灵活方法。在这里，我们创造了一个被称为“转基因之战”的概念，基于此，我们使用多个重组酶的基因工程之战来产生策略。这些策略使得能够对多个转基因进行分裂可调的分配。我们证明了这些策略和技术在诱导多个转基因的强和多稀疏分配中的多功能性。此外，我们的转基因策略和扩展显微镜的组合，使三维高分辨率成像的整个突触结构在海马体与内源性突触蛋白的同时可视化。这些基于基因之战的策略和技术可能会加速在不同生命科学领域中对整个突触和细胞连接的分析。
Elucidating fine architectures and functions of cellular and synaptic connections requires development of new flexible methods. Here, we created a concept called the "battle of transgenes," based on which we generated strategies using genetically engineered battles of multiple recombinases. The strategies enabled split-tunable allocation of multiple transgenes. We demonstrated the versatility of these strategies and technologies in inducing strong and multi-sparse allocations of multiple transgenes. Furthermore, the combination of our transgenic strategy and expansion microscopy enabled three-dimensional high-resolution imaging of whole synaptic structures in the hippocampus with simultaneous visualizations of endogenous synaptic proteins. These strategies and technologies based on the battle of genes may accelerate the analysis of whole synaptic and cellular connections in diverse life science fields.