Multiple cis-acting elements modulate the translational efficiency of GCN4 mRNA in yeast.
Multiple cis-acting elements modulate the translational efficiency of GCN4 mRNA in yeast.
复制标题
多个顺式作用元件调节酵母中 GCN4 mRNA 的翻译效率。
DOI：
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发表时间：
1986
影响因子：
11.1
通讯作者：
G. Thireos
中科院分区：
文献类型：
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作者：
D. Tzamarias;D. Alexandraki;G. Thireos
酵母中 GCN4 基因的表达在翻译水平上受到调节：酵母细胞在氨基酸饥饿条件下的生长导致 GCN4 mRNA 翻译效率的增加。该 mRNA 5' 非翻译区内的序列包含四个小的开放阅读框，当在丰富的培养基中生长时，该序列以顺式作用抑制翻译。在本报告中，我们分析了GCN4 mRNA 5'非翻译区的一系列缺失、插入和替换突变对翻译的影响。该分析表明，GCN4 mRNA 的翻译激活需要位于小型上游开放阅读框区域内的至少两个不同的顺式元件以及反式阳性元件。我们提出 GCN4 mRNA 的翻译效率受上游 AUG 密码子翻译起始速率的调节。
The expression of the GCN4 gene in yeast is regulated at the translational level: growth of yeast cells under amino acid starvation conditions results in an increase in the translational efficiency of GCN4 mRNA. A sequence within the 5' untranslated region of this mRNA, which contains four small open reading frames, acts in cis to suppress translation when growth occurs in rich media. In this report, we have analyzed the effects on translation of a series of deletion, insertion, and substitution mutations in the 5' untranslated region of GCN4 mRNA. This analysis showed that at least two distinct cis elements located within the region of the small upstream open reading frames are required, in conjunction with trans positive elements, for the translational activation of GCN4 mRNA. We propose that the translational efficiency of GCN4 mRNA is modulated by the rate of translation initiation at the upstream AUG codons.