Tracheary elements from calli of Japanese horse chestnut (Aesculus turbinata) form perforation-like structures
Tracheary elements from calli of Japanese horse chestnut (Aesculus turbinata) form perforation-like structures
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日本七叶树（七叶树）愈伤组织的气管成分形成穿孔状结构
DOI：
10.1007/s00425-021-03621-4
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发表时间：
2021
期刊：
影响因子：
4.3
通讯作者：
Funada Ryo
中科院分区：
文献类型：
--
作者：
Yamagishi Yusuke;Kudo Kayo;Yoshimoto Joto;Nakaba Satoshi;Nabeshima Eri;Watanabe Ugai;Funada Ryo
主要结论：七叶树幼叶愈伤组织中含有导管分子，导管分子具有类似导管分子穿孔的大孔，导管分子的离体分化为详细分析木质部细胞分化提供了有用的系统。为了研究细胞壁结构形成的机制，需要新的分化系统，使我们能够诱导高度组织化的结构，如穿孔。在这项研究中，我们开发了这样一个系统，在其中我们能够诱导形成的导管分子与穿孔，使用愈伤组织的硬木，七叶树turbinata。A.将小鼻甲骨接种在含有5 μM 2，4-D和5 μM BA的改良MS培养基上。从紫云英幼叶愈伤组织中诱导出导管分子。鼻甲骨一些导管分子形成了具有典型次生木质部特征的次生壁。其他导管分子形成螺旋状加厚，这是次生木质部导管分子的典型特征。约10%的导管分子形成类似导管分子穿孔的大孔，并观察到各种类型的穿孔板。在诱导培养基中添加NAA和油菜素可促进愈伤组织导管分子的分化。鼻甲骨新诱导的导管分子也形成了次生木质部的典型特征，如导管分子的穿孔。我们的模型系统可能是有用的努力，以了解在次生木质部中的管分子的高度有组织的结构的形成机制。
Main conclusionCalli derived from young leaves ofAesculus turbinatacontained tracheary elements with large pores that resembled perforations of vessel elements.AbstractThe differentiation of tracheary elements in vitro provides a useful system for detailed analysis of xylem cell differentiation. To examine the mechanism of formation of cell wall structures, new differentiation systems are required that allows us to induce highly organized structures, such as perforations. In this study, we developed such a system in which we were able to induce formation of tracheary elements with perforations, using calli of a hardwood,Aesculus turbinata. Young leaves ofA. turbinatawere placed on modified MS medium that contained 5 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 5 μM benzyladenine (BA). Tracheary elements were induced in calli derived from young leaves ofA. turbinata. Some tracheary elements formed broad areas of secondary wall with typical features of secondary xylem. Other tracheary elements formed spiral thickenings, which are typical features of vessel elements in secondary xylem ofA. turbinata.Approximately 10% of tracheary elements formed large pores that resembled perforations of vessel elements and various types of the perforation plate were observed. Addition of NAA and brassinolide to the induction medium enhanced the differentiation of tracheary elements in calli ofA. turbinata. Newly induced tracheary elements also formed typical features of secondary xylem such as perforations of the vessel elements. Our model system might be useful in efforts to understand the mechanisms of formation of highly organized structures in tracheary elements in secondary xylem.