Transforming c-Ki-ras mutation is a preneoplastic event in mouse mammary carcinogenesis induced in vitro by N-methyl-N-nitrosourea.
Transforming c-Ki-ras mutation is a preneoplastic event in mouse mammary carcinogenesis induced in vitro by N-methyl-N-nitrosourea.
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转化c-Ki-ras突变是N-甲基-N-亚硝基脲体外诱导的小鼠乳腺癌发生过程中的一个癌前事件。
DOI：
10.1128/mcb.10.4.1593-1599.1990
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发表时间：
1990
影响因子：
5.3
通讯作者：
Nandi,S
中科院分区：
文献类型：
--
作者：
Miyamoto,S;Sukumar,S;Guzman,RC;Osborn,RC;Nandi,S
小鼠乳腺上皮细胞经N-甲基-N-亚硝基脲（MNU）处理后，可在原代培养中转化为癌前状态和肿瘤状态。由MNU诱导的增生性肺泡结节（一种小鼠乳腺癌前病变）引起的乳腺癌在NIH 3 T3焦点试验中检测时含有转化c-Ki-ros基因。等位基因特异性寡核苷酸与聚合酶链反应扩增的c-Ki-ros序列的杂交表明，NIH 3 T3病灶和乳腺癌中均存在密码子12处的特异性G-35→A-35点突变。这种突变导致正常的甘氨酸被天冬氨酸取代。此外，在10个增生性肺泡结节中的9个中也检测到c-Ki-ras原癌基因的突变。这些结果表明，特定的c-Ki-ras突变是MNU诱导的小鼠乳腺癌发生的癌前事件。
Mouse mammary epithelial cells can be transformed in primary cultures to preneoplastic and neoplastic states when treated withN-methyl-N-nitrosourea (MNU). Mammary carcinomas arising from MNU-induced hyperplastic alveolar nodules (a type of mouse mammary preneoplastic lesion) contained transforming c-Ki-ros genes when examined by the NIH 3T3 focus assay. Hybridization of allele-specific oligonucleotides to c-Ki-ros sequences amplified by the polymerase chain reaction demonstrated the presence of a specific G-35→A-35 point mutation in codon 12 in each of the NIH 3T3 foci as well as the mammary carcinomas. This mutation resulted in the substitution of the normal glycine with an aspartic acid. Furthermore, this mutation in the c-Ki-rasproto-oncogenes was also detected in 9 of 10 hyperplastic alveolar nodules. These results demonstrate that the specific c-Ki-rasmutation is a preneoplastic event in MNU-induced mouse mammary carcinogenesis.