Terahertz Spectroscopy of Tetrameric Peptides
Terahertz Spectroscopy of Tetrameric Peptides
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DOI：
10.1021/acs.jpclett.9b01091
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发表时间：
2019-05-16
影响因子：
5.7
通讯作者：
Schmuttenmaer, Charles A.
中科院分区：
文献类型：
--
作者：
Neu, Jens;Stone, Elizabeth A.;Schmuttenmaer, Charles A.
确定多肽的序列和结构对于理解它们的结构-性质关系是至关重要的。在许多技术中，通常使用核磁共振光谱和单晶X射线衍射测量来确定结构。在这项研究中，我们提出了太赫兹时域光谱(THz-TDS)作为一种补充的、非破坏性的技术，它对四肽的一级和二级结构都很敏感。仅使用几毫克肽，就测量了THz-TDS光谱，其中一些光谱得到了密度泛函理论(DFT)的支持，以区分六个具有相似一级和二级结构的四聚体多肽。
Determining the sequence and structure of peptides is crucial for understanding their structure-property relationships. Among many techniques, structures are typically elucidated using nuclear magnetic resonance spectroscopy and single crystal X-ray diffraction measurements. In this study, we present terahertz time-domain spectroscopy (THz-TDS) as a complementary, non-destructive technique that is sensitive to both the primary and secondary structures of tetrapeptides. Using only a few milligrams of peptide, THz-TDS spectra have been measured, some of which have been supported by density functional theory (DFT) calculations, to distinguish six tetrameric peptides with similar primary and secondary structures.