Construction of an extended range whole-cell tetracycline biosensor by use of the tet(M) resistance gene
Construction of an extended range whole-cell tetracycline biosensor by use of the tet(M) resistance gene
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DOI：
10.1016/j.femsle.2005.09.034
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发表时间：
2005-12-15
影响因子：
2.1
通讯作者：
Sorensen, SJ
中科院分区：
文献类型：
--
作者：
Bahl, MI;Hansen, LH;Sorensen, SJ
将四环素抗性核糖体保护编码基因tet(M)插入含有四环素调控启动子和绿色荧光蛋白基因转录融合的pTGFP2载体中，构建了四环素全细胞生物传感器菌株。四环素、土霉素、金环素和米诺环素均能有效地诱导大肠杆菌MC4100/pTGM生物传感器的形成，流式细胞仪检测结果显示四种化合物的剂量-反应特性相似。新的四环素生物传感器对药物浓度的响应范围从低于5ngml(-1)到16mgml(-1)，这是对原始版本的显着改进。(C)2005年欧洲微生物学会联合会。爱思唯尔出版，版权所有。
An extended range whole-cell tetracycline biosensor strain was constructed by insertion of the tet(M) gene, encoding tetracycline resistance by ribosomal protection, into plasmid pTGFP2, which contains a transcriptional fusion between a tetracycline regulated promoter and the green fluorescent protein gene. Tetracycline, oxytetracycline, chlortetracycline and minocycline all effectively induced the resulting Escherichia coli MC4100/pTGM biosensor and similar dose-response characteristics were recorded by flow cytometry for all four compounds. The novel tetracycline biosensor was responsive to drug concentrations ranging from below 5 ng ml(-1) to 16 mu g ml(-1) which represents a significant improvement of the original version. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.