Multilocus phylogenetic structure within the Trichoderma harzianum/Hypocrea lixii complex
Multilocus phylogenetic structure within the Trichoderma harzianum/Hypocrea lixii complex
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DOI：
10.1016/s1055-7903(02)00400-1
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发表时间：
2003-05-01
影响因子：
4.1
通讯作者：
Geiser, DM
中科院分区：
文献类型：
--
作者：
Chaverri, P;Castlebury, LA;Geiser, DM
哈茨木霉是一种广泛存在于环境中的真菌，在植物病原真菌的生物防治中具有重要作用。T harzianum没有明确地联系到它的性状态，也没有详细研究它的生殖发育。有人认为，哈茨木霉是一个复杂的物种的基础上遇到的表型和基因型变异。根据形态学和培养特征以及ITS rDNA、翻译延伸因子1-α、钙调蛋白和α-肌动蛋白4个基因的DNA序列分析，认为利氏肉座菌是哈茨木霉的有性状态。该物种的无性和有性状态具有广泛的地理分布。系统发育分析表明，哈茨木霉/H. LixII是由高于95%的自举值支持的内聚基团。根据系谱一致性原则，认为T harzianum/H. lixii是一个复杂的独立的单系谱系，但没有诊断形态学的区别被确定，证明正式的分类识别的不同谱系。(C)2002 Elsevier Science（美国）。All rights reserved.
Trichoderma harzianum is a ubiquitous species in the environment and is effective in the biological control of plant-pathogenic fungi. T harzianum has not been linked unequivocally to its sexual state nor has its phylogeny been studied in detail. It has been suggested that T harzianum is a species complex based on the phenotypic and genotypic variability encountered. On the basis of morphological and cultural characters and DNA sequence data analysis of four genes (ITS rDNA, translation elongation factor 1-alpha, calmodulin, and alpha-actin), Hypocrea lixii was found to be the sexual state of T harzianum. Both the asexual and sexual states of this species have wide geographic distributions. Phylogenctic analysis of four genes showed that T harzianum/H. lixii is a cohesive group that is supported by bootstrap values higher than 95%. Principles of genealogical concordance indicated that T harzianum/H. lixii is a complex of independent monophyletic lineages, but no diagnostic morphological distinctions were identified that justify formal taxonomic recognition for the different lineages. (C) 2002 Elsevier Science (USA). All rights reserved.