Excision-amplification of mitochondrial DNA during senescence in Podospora anserina. A potential role for an 11 base-pair consensus sequence in the excision process.
Excision-amplification of mitochondrial DNA during senescence in Podospora anserina. A potential role for an 11 base-pair consensus sequence in the excision process.
复制标题
鹅足孢衰老过程中线粒体 DNA 的切除-扩增。
DOI：
10.1016/0022-2836(87)90304-4
复制
发表时间：
1987
影响因子：
5.6
通讯作者：
Cummings,DJ
中科院分区：
文献类型：
--
作者：
Turker,MS;Domenico,JM;Cummings,DJ
鉴定了三种新的鹅足孔菌线粒体切除扩增质粒，并确定了线粒体基因组上的切除连接位点。所有三个质粒至少部分源自线粒体基因组的一个共同区域，称为EcoRI-7 (E7)。给出了 E7 的整个 5651 个碱基对序列。该序列中包括这些新型质粒的 E7 特异性切除连接位点、九个 tRNA 基因的定位以及大 rRNA 线粒体基因的 I 类内含子的定位。 E7 区域包含该大 rRNA 基因的 3' 部分。发现这三种新型质粒以及其他先前描述的线粒体质粒的形成与 11 碱基对共有序列 GGCGCAAGCTC 或其互补序列的存在有关。讨论了该共有序列及其互补序列在质粒形成和足孢菌衰老过程中的可能作用。考虑了 tRNA 基因在质粒形成中的可能作用。
Three novel mitochondrial excision-amplification plasmids ofPodonpora anserinawere identified and the excision-junction sites on the mitochondrial genome determined. All three plasmids were at least partially derived from a common region of the mitochondrial genome termedEcoRI-7 (E7). The entire 5651 base-pair sequence of E7 is presented. Included within this sequence are the E7-specific excision-junction sites of these novel plasmids, the localizations of nine tRNA genes, and the localization of a class I intron of the large rRNA mitochondrial gene. The E7 region contains the 3′ portion of this large rRNA gene. Formation of these three novel plasmids as well as other previously described mitochondrial plasmids was found to be associated with the presence of an 11 base-pair consensus sequence, GGCGCAAGCTC, or its complementary sequence. A possible role for this consensus sequence and its complement in plasmid formation and the senescence process ofPodosporais discussed. A possible role for the tRNA genes in plasmid formation is considered.