Functional interchangeability of DNA replication genes in Salmonella typhimurium and Escherichia coli demonstrated by a general complementation procedure.
Functional interchangeability of DNA replication genes in Salmonella typhimurium and Escherichia coli demonstrated by a general complementation procedure.
复制标题
通过一般互补程序证明了鼠伤寒沙门氏菌和大肠杆菌中 DNA 复制基因的功能互换性。
DOI：
10.1093/genetics/108.1.1
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发表时间：
1984
期刊：
影响因子：
3.3
通讯作者：
Botstein,D
中科院分区：
文献类型：
--
作者：
Maurer,R;Osmond,BC;Shekhtman,E;Wong,A;Botstein,D
利用溶原性互补技术从λ-沙门氏菌基因组文库中分离到24个影响DNA复制的基因。大肠杆菌，使用一种新的空斑互补试验。互补λ克隆（在该试验中产生红色噬斑）和非互补突变衍生物（产生无色噬斑）用于进一步表征温度敏感沙门氏菌突变体，并建立E.大肠杆菌和沙门氏菌DNA复制基因。17、18E在代表不同位点的共突变体中，发现了与突变体互补的沙门氏菌基因，这一结果表明，单个沙门氏菌复制蛋白能够在其他等位基因中起作用。大肠杆菌复制复合物，并表明沙门氏菌和E.大肠杆菌复制蛋白非常相似。另外7个被克隆的沙门氏菌基因在功能上与任何E都不相关。大肠杆菌检查。- 作为影响一些克隆基因的染色体突变的衍生的辅助手段，开发了一种通用方法，用于将转座子置于沙门氏菌染色体中对应于克隆DNA的区段中。沙门氏菌中的染色体突变影响了一个基因（dnaA），该基因是通过一个E的互补克隆的。通过使用转座子编码的药物抗性作为选择标记在局部诱变中获得共突变体。
Twenty-four genes fromSalmonella typhimuriumthat affect DNA replication were isolated from a λ-Salmonella genomic library by lysogenic complementation of temperature-sensitive mutants of Salmonella orE. coli, using a new plaque complementation assay. The complementing λ clones, which make red plaques in this assay, and noncomplementing mutant derivatives, which make uncolored plaques, were used to further characterize the temperature-sensitive Salmonella mutants and to establish the functional similarity ofE. coliand Salmonella DNA replication genes. For 17 of 18E. colimutants representing distinct loci, a Salmonella gene that complemented the mutant was found. This result indicates that single Salmonella replication proteins are able to function in otherwise allE. colireplication complexes and suggests that the detailed properties of Salmonella andE. colireplication proteins are very similar. The other seven Salmonella genes that were cloned were unrelated functionally to anyE. coligenes examined. —As an aid to the derivation of chromosomal mutations affecting some of the cloned genes, a general method was developed for placing a transposon in the Salmonella chromosome in a segment corresponding to cloned DNA. Chromosomal mutations were derived in Salmonella affecting a gene (dnaA) that was cloned by complementation of anE. colimutant by using the transposon-encoded drug resistance as a selectable marker in local mutagenesis.