The influence of thymoquinone on doxorubicin-induced hyperlipidemic nephropathy in rats
The influence of thymoquinone on doxorubicin-induced hyperlipidemic nephropathy in rats
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DOI：
10.1016/s0300-483x(99)00179-1
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发表时间：
2000-03-07
期刊：
影响因子：
4.5
通讯作者：
Hamada, FMA
中科院分区：
文献类型：
--
作者：
Badary, OA;Abdel-Naim, AB;Hamada, FMA
研究了黑种草子挥发油的主要成分百里醌（TQ）对阿霉素（DOX）所致大鼠肾病及氧化应激的影响。单次静脉注射DOX（6 mg/kg）诱导严重肾病综合征（5周后），其与低白蛋白血症、低蛋白血症、血清尿素升高、高脂血症以及蛋白质、白蛋白和N-乙酰基-β-D-氨基葡萄糖苷酶（NAG）的高尿排泄相关。在肾脏中，DOX诱导总甘油三酯（TG）、总胆固醇（TC）和脂质过氧化物显著增加，非蛋白巯基（NPSH）含量和过氧化氢酶（CAT）活性显著降低。TQ（10 mg/kg/天）给药前5天和以后每天补充饮用水，可显著降低血清尿素、TG和TC。与DOX单用组相比，TQ治疗组大鼠肾脏中TG、TC和脂质过氧化物水平均显著降低。与单纯DOX组相比，TQ-DOX组大鼠肾脏NPSH含量和CAT活性显著升高。TQ治疗显著抑制DOX诱导的蛋白尿。蛋白尿和尿NAG排泄。结果证实自由基参与DOX诱导的肾病的发病机制。同样，该研究表明TQ的高抗氧化潜力及其对抑制DOX诱导的肾病的显著作用。提示TQ可作为肾病综合征蛋白尿和高脂血症的保护剂。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
The effect of thymoquinone (TQ), the main constituent of the volatile oil of Nigella sativa seeds, on the nephropathy and oxidative stress induced by doxorubicin (DOX) in rats was investigated. A single intravenous injection of DOX (6 mg/kg) induced a severe nephrotic syndrome (after 5 weeks) associated with hypoalbuminemia, hypoproteinemia, elevated serum urea, hyperlipidemia, and a high urinary excretion of protein, albumin and N-acetyl-beta-D-glucosaminidase (NAG). In the kidney, DOX induced a significant increase in total triglycerides (TG), total cholesterol (TC), and lipid peroxides and a significant decrease in non-protein sulfhydryl (NPSH) content and catalase (CAT) activity. Treatment of rats with TQ (10 mg/kg per day) supplemented with the drinking water for 5 days before DOX, and daily thereafter, significantly lowered serum urea, TG, and TC. Similarly, TG, TC and lipid peroxides in the kidneys of TQ-treated rats were decreased significantly compared with DOX alone. Moreover, NPSH content and CAT activity in the kidneys of TQ-treated DOX group were significantly elevated compared with DOX alone. Treatment with TQ significantly suppressed DOX-induced proteinuria. albuminuria, and urinary excretion of NAG. The results confirm the involvement of free radicals in the pathogenesis of nephropathy induced by DOX. Likewise, the study demonstrates the high antioxidant potential of TQ and its marked effect on the suppression of DOX-induced nephropathy. The data suggest that TQ might be applicable as a protective agent for proteinuria and hyperlipidemia associated with nephrotic syndrome. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.