Polymorphism of SFBB−γ and its use for S genotyping in Japanese pear (Pyrus pyrifolia)
Polymorphism of SFBB−γ and its use for S genotyping in Japanese pear (Pyrus pyrifolia)
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SFBB−γ 多态性及其在日本梨 (Pyruspyrifolia) S 基因分型中的应用
DOI：
10.1007/s00299-008-0619-5
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发表时间：
2008
影响因子：
6.2
通讯作者：
H. Sassa
中科院分区：
文献类型：
--
作者：
H. Kakui;Takeshi Tsuzuki;T. Koba;H. Sassa
日本梨（Pyrus pyrifolia）表现出基于S-RNase的配子体自交不亲和性，其中花粉部分决定因子pollenS长期以来一直是难以捉摸的。最近在日本梨和苹果中鉴定出的Slocus F-box兄弟（SFBB）表明，多个F-box基因具有花粉特异性表达、形态特异性多态性和与Slocus的连锁性，是花粉Slocus的候选基因。在日本梨中，从一个单倍型中鉴定出三个SFBB，并且它们与同组的其他单倍型基因（即，α-、β-和γ-基团）。在本研究中，我们从日本梨的不同S基因型中分离了7个新的PpSFBB −γ基因。这些基因均表现出单体型特异性多态性，但序列相似性很高。基于PpSFBB −γ基因的序列多态性，我们开发了一个CAPS/dCAPS系统，用于日本梨品种的S基因分型。新的S基因分型系统不仅能区分S1-S9，而且适用于自交亲和品种选育中的M_4sm单倍型鉴定和Sk等新单倍型的检测。
Japanese pear (Pyrus pyrifolia) exhibits the S-RNase-based gametophytic self-incompatibility where the pollen-part determinant, pollenS, had long remained elusive. Recent identification ofSlocus F-box brothers (SFBB) in Japanese pear and apple suggested that the multiple F-box genes are the pollenScandidates as they exhibited pollen specific expression,Shaplotype-specific polymorphisms and linkage to theSlocus. In Japanese pear, threeSFBBs were identified from a singleShaplotype, and they were more homologous to other haplotype genes of the same group (i.e., α-, β- and γ-groups). In this study, we isolated new sevenPpSFBB−γgenes from differentSgenotypes of Japanese pear. These genes showedShaplotype-specific polymorphisms, however, sequence similarities among them were very high. Based on the sequence polymorphisms of thePpSFBB−γgenes, we developed a CAPS/dCAPS system forSgenotyping of the Japanese pear cultivars. This newSgenotyping system was found to not only be able to discriminate theS1–S9, but also be suitable for identification of the mutantS4smhaplotype for the breeding of self-compatible cultivars, and detection of newShaplotypes such asSk.