Quantitation of base substitutions and deletions induced by chemical mutagens during DNA synthesis in vitro.
Quantitation of base substitutions and deletions induced by chemical mutagens during DNA synthesis in vitro.
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体外 DNA 合成过程中化学诱变剂诱导的碱基取代和缺失的定量。
DOI：
10.1021/tx00035a006
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发表时间：
1993
影响因子：
4.1
通讯作者：
Shibutani,S
中科院分区：
文献类型：
--
作者：
Shibutani,S
材料和方法。用于合成寡脱氧核苷酸的有机化学品由Aldrich Chemical（密尔沃基，WI）提供。乙腈、三乙胺和蒸馏水（均为HPLC级）购自Fisher Chemical（Pittsburgh，PA）。[7- 32 P] ATP（比活度> 5000 Ci/mmol）获自阿默舍姆Corp.。（阿灵顿高地，IL）。大肠杆菌DNA聚合酶I的克隆的无3 ′-5 ′核酸外切酶（exo-）（21200单位/mg蛋白质）和完整的（exo+）（17400单位/mg）Klenow片段购自美国生物化学公司。（Cleveland，OH）;完整DNA
Materials and Methods. Organic chemicals used for the synthesis of oligodeoxynucleotides were supplied by Aldrich Chemical (Milwaukee, WI). Acetonitrile, triethylamine, and distilled water, allHPLC grade, were purchased from Fisher Chemical (Pittsburgh, PA).[7-32P] ATP (specific activity> 5000 Ci/mmol) was obtained from Amersham Corp.(Arlington Heights, IL). Cloned 3'-5'exonuclease-free (exo-)(21 200 units/mg of protein) and intact (exo+)(17 400 units/mg) Klenow fragments of Escherichia coli DNA polymerase I were purchased from United States Biochemical Corp.(Cleveland, OH); intact DNA