THE EFFECT OF POST-BLOODMEAL NUTRITION OF PHLEBOTOMUS-PAPATASI ON THE TRANSMISSION OF LEISHMANIA-MAJOR
THE EFFECT OF POST-BLOODMEAL NUTRITION OF PHLEBOTOMUS-PAPATASI ON THE TRANSMISSION OF LEISHMANIA-MAJOR
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DOI：
10.4269/ajtmh.1986.35.926
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发表时间：
1986-09-01
影响因子：
3.3
通讯作者：
SCHLEIN, Y
中科院分区：
文献类型：
--
作者：
WARBURG, A;SCHLEIN, Y
为探讨沙蝇餐后营养对大白鼠利什曼原虫传播的影响，以蔗糖、海藻糖、白蛋白或蔗糖和白蛋白的混合物为饲料，对感染白纹伊蚊的雌性白纹伊蚊进行毛细管强制喂养。通过计算排出的寄生虫来评估传播情况；寄生虫的数量从0到1000多个前鞭毛体不等。所有试验组的大多数果蝇都感染了前中肠，但在以蔗糖和白蛋白的混合物维持的组中，传播雌性果蝇的比例明显更高。在苍蝇的咽部和烟囱中没有附着的寄生虫，这些部位存在游离的前鞭毛体本身并不表明具有传染性。然而，传播与明显的吞噬能力呈正相关。排出的寄生虫是典型的感染性前鞭毛虫，与在食道和胸前中肠观察到的寄生虫相同。提出了一种来自食道和口瓣的感染期前鞭毛体可能通过咬合传播的机制。
To test the effects of post-bloodmeal nutrition of sand flies on the transmission of Leishmania major, groups of infected Phlebotomus papatasi females maintained on diets of sucrose, trehalose, albumin or a mixture of sucrose and albumin, were subjected to forced feeding with capillaries. Transmission was evaluated by counting the parasites egested; numbers ranged from 0 to over 1,000 promastigotes. Infections of the anterior midgut were seen in the majority of flies from all the experimental groups but the percentage of transmitting females was significantly higher in the group maintained on a mixture of sucrose and albumin. There were no attached parasites in the pharynx and cibarium of the flies and the presence of free promastigotes in these parts was not itself indicative of infectivity. However, transmission was positively correlated with apparent inability to engorge. The parasites egested were typical infective form promastigotes and identical to those observed in the esophagus and the anterior thoracic midgut. A mechanism by which infective stage promastigotes from the esophagus and the stomodeal valve may be transmitted by bite is proposed.