Modification of silk fibroin using diazonium coupling chemistry and the effects on hMSC proliferation and differentiation
Modification of silk fibroin using diazonium coupling chemistry and the effects on hMSC proliferation and differentiation
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DOI：
10.1016/j.biomaterials.2008.03.039
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发表时间：
2008-07-01
期刊：
影响因子：
14
通讯作者：
Kaplan, David L.
中科院分区：
文献类型：
--
作者：
Murphy, Amanda R.;John, Peter St.;Kaplan, David L.
开发了一种利用重氮偶联化学的简单化学修饰方法，以调整丝素蛋白的结构和亲水性。使用几种苯胺衍生物的修饰程度通过紫外 - 可见光谱和氢 - 1核磁共振光谱进行了表征，并且使用衰减全反射 - 傅里叶变换红外光谱分析了所得的蛋白质结构。疏水官能团的引入促进了蛋白质从无规卷曲到β - 折叠结构的快速转变，而亲水基团的添加抑制了这一过程。人骨髓间充质干细胞（hMSCs）在这些修饰的丝素材料上生长，以评估这些材料的生物相容性。发现丝素衍生物的亲水性影响生长速率和形态，但人骨髓间充质干细胞能够在所有丝素衍生物上附着、增殖并分化为成骨细胞谱系。（C）2008爱思唯尔有限公司。保留所有权利。
A simple chemical modification method using diazonium coupling chemistry was developed to tailor the structure and hydrophilicity of silk fibroin protein. The extent of modification using several aniline derivatives was characterized using UV-vis and H-1 NMR spectroscopies, and the resulting protein structure was analyzed with ATR-FTIR spectroscopy. Introduction of hydrophobic functional groups facilitated rapid conversion of the protein from a random coil to a P-sheet structure, while addition of hydrophilic groups inhibited this process. hMSCs were grown on these modified silks to assess the biocompatibility of these materials. The hydrophilicity of the silk derivatives was found to affect the growth rate and morphology, but hMSCs were able to attach, proliferate and differentiate into an osteogenic lineage on all of the silk derivatives. (C) 2008 Elsevier Ltd. All rights reserved.