Adipogenic, osteogenic and myofibrogenic differentiations of a rat malignant fibrous histiocytoma (MFH)-derived cell line, and a relationship of MFH cells with embryonal mesenchymal, perivascular and bone marrow stem cells.
Adipogenic, osteogenic and myofibrogenic differentiations of a rat malignant fibrous histiocytoma (MFH)-derived cell line, and a relationship of MFH cells with embryonal mesenchymal, perivascular and bone marrow stem cells.
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DOI：
10.1016/j.ejca.2007.10.021
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发表时间：
2007-12
影响因子：
8.4
通讯作者：
J. Yamate;K. Ogata;T. Yuasa;M. Kuwamura;S. Takenaka;D. Kumagai;K. Itoh;J. LaMarre
中科院分区：
文献类型：
--
作者：
J. Yamate;K. Ogata;T. Yuasa;M. Kuwamura;S. Takenaka;D. Kumagai;K. Itoh;J. LaMarre
恶性纤维组织细胞瘤（MFH）被认为是一种未分化的多形性肉瘤，其组织发生尚未得到证实。我们研究了大鼠 MFH 细胞系 (MT-9) 的病理生物学特征。免疫细胞化学显示，MT-9 细胞和 MT-9 诱导的肿瘤对波形蛋白、A3（大鼠 MFH 细胞特异性抗体）、巨噬细胞标记物和 α-SMA（肌成纤维细胞标记物）发生反应，表明 MT-9 显示组织细胞和（肌）成纤维细胞特征。添加成脂补充剂的 MT-9 显示脂滴积累增加。在 MT-9 中添加 BMP-2 或成骨补充剂可增强成骨细胞标志物（ALP 活性、骨钙素 mRNA 表达和钙化）。 TGF-β1 处理的 MT-9 显示 α-SMA 免疫阳性细胞数量增加，α-SMA 和纤连蛋白蛋白水平增强，表明肌纤维形成。在大鼠组织中，A3 在胎儿和新生儿中标记未成熟的间充质和血管周围细胞，在成人中标记骨髓干细胞。在骨髓和 MT-9 中观察到 c-kit mRNA 表达。总的来说，MFH 祖细胞应该在能够分化为间充质细胞的骨髓干细胞谱系中寻找。
Malignant fibrous histiocytoma (MFH) is regarded as an undifferentiated pleomorphic sarcoma with unproven histogenesis. We investigated pathobiological characteristics of a rat MFH cell line (MT-9). Immunocytochemically, MT-9 cells and MT-9-induced tumours reacted to vimentin, A3 (rat MFH cell-specific antibody), macrophage markers and α-SMA (myofibroblastic marker), indicating that MT-9 showed both histiocytic and (myo)fibroblastic features. Adipogenic supplement-added MT-9 showed increased accumulation of lipid droplets. Addition of BMP-2 or osteogenic supplement to MT-9 enhanced osteoblastic markers (ALP activity, osteocalcin mRNA expression and calcification). TGF-β1-treated MT-9 revealed increased numbers of α-SMA-immunopositive cells, and enhanced protein levels of α-SMA and fibronectin, indicating myofibrogenesis. In rat tissues, A3 labelled with immature mesenchymal and perivascular cells in foetuses and neonates, and with marrow stem cells in adults. c-kit mRNA expression was seen in bone marrows and MT-9. Collectively, progenitors of MFH should be sought in lineage of marrow stem cells capable of differentiating into mesenchymal cells.