Neurokinin (NK)-1 receptor expression in monocytes from bipolar disorder patients: A pilot study
Neurokinin (NK)-1 receptor expression in monocytes from bipolar disorder patients: A pilot study
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DOI：
10.1016/j.jad.2015.03.013
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发表时间：
2015-06-01
影响因子：
6.6
通讯作者：
Brunelleschi, Sandra
中科院分区：
文献类型：
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作者：
Amoruso, Angela;Bardelli, Claudio;Brunelleschi, Sandra
背景：神经激肽 1 受体 (NK-1R) 与包括重度抑郁症在内的多种精神疾病有关，但对于双相情感障碍 (BD) 知之甚少。方法：我们比较了 BD 患者和健康供体 (HD) 的单核细胞中 NK-1R 的表达和 P 物质 (SP) 诱导 NF-κ B 激活的能力，同时也寻找烟草烟雾的影响。在签署知情书面同意书后，20 名正常 BD 患者（1 型双相情感障碍 (BDI) 或 2 型双相情感障碍 (BDII)）和 14 名年龄匹配的健康捐赠者 (HD) 入组。通过蛋白质印迹评估单核细胞中的 NK-1R 表达，并表示为 NK-1R 与 Na+/K+-ATPase 蛋白表达之间的比率。通过测量 p50 亚基的核含量（ELlSA 试剂盒）来评估 NF-kappa B 激活。结果：NK-1R 表达显着降低（P
Background: Neurokinin 1 receptors (NK-1R) have been involved in several psychiatric disorders including major depression, but less is known for bipolar disorder (BD).Method: We compared NK-1R expression and Substance P (SP) ability to induce NF-kappa B activation in monocytes from BD patients and healthy donors (HD), also looking for the effects of tobacco smoke. After informed written consent, 20 euthymic BD patients, either bipolar type 1 (BDI) or type 2 (BDII), and 14 age matched healthy donors (HD) were enrolled. NK-1R expression in monocytes was evaluated by Western blot and expressed as the ratio between NK-1R and Na+/K+-ATPase protein expressions. NF-kappa B activation was assessed by measuring the nuclear content of the p50 subunit (ELlSA kit).Results: NK-1R expression was significantly reduced (P