Bacillus amyloliquefaciens PDR1 from root of karst adaptive plant enhances Arabidopsis thaliana resistance to alkaline stress through modulation of plasma membrane H+-ATPase activity
Bacillus amyloliquefaciens PDR1 from root of karst adaptive plant enhances Arabidopsis thaliana resistance to alkaline stress through modulation of plasma membrane H+-ATPase activity
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来自喀斯特适应性植物根部的解淀粉芽孢杆菌 PDR1 通过调节质膜 H -ATP 酶活性增强拟南芥对碱性胁迫的抵抗力
DOI：
10.1016/j.plaphy.2020.08.019
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发表时间：
2020-10-01
影响因子：
6.5
通讯作者：
Yi, Yin
中科院分区：
文献类型：
--
作者：
Li, Fei;Shi, Tianlong;Yi, Yin
探索原生微生物是开发生态修复微生物制剂的可行途径。本研究旨在探讨喀斯特适应性植物解淀粉芽孢杆菌PDR1对拟南芥根质膜H+- atp酶活性的影响。在有解淀粉芽孢杆菌PDR1或不存在PDR1的条件下培养拟南螺旋藻，并通过测定主根长度和干重来评价其对生长的影响。采用pH指示剂、pH计和非侵入性微试验技术（NMT）检测根际酸化能力。采用适当的方法进行养分吸收。采用转录组测序和实时定量聚合酶链反应（qRT-PCR）相结合的方法检测拟南拟南根质膜H+- atp酶活性调控功能基因的表达。通过功能分析了解解淀粉芽孢杆菌如何调节生物过程和代谢途径，增强拟南芥对碱性胁迫的抗性。本研究表明，解淀粉芽孢杆菌PDR1挥发性有机物（VOCs）促进拟南芥的生长发育，增强质膜H+- atp酶活性，影响拟南芥的离子吸收。此外，解淀粉芽孢杆菌PDR1 VOCs不影响质膜H+-ATPase编码基因的表达，但影响了调节质膜H+-ATPase活性的基因的表达。本研究结果阐明了解淀粉芽孢杆菌调控拟南芥生长和碱胁迫抗性的机制，为其在农业生产和生态保护中的广泛有效应用奠定了基础。
Exploration of native microbes is a feasible way to develop microbial agents for ecological restoration. This study was aimed to explore the impact of Bacillus amyloliquefaciens PDR1 from karst adaptive plant on the activity of root plasma membrane H+-ATPase in Arabidopsis thaliana. A. thaliana was cultured in presence or absence of B. amyloliquefaciens PDR1 and its effects on the growth were evaluated by measuring the taproot length and dry weight. The rhizosphere acidification capacity was detected by a pH indicator, a pH meter and non-invasive micro-test techniques (NMT). The nutrient uptake was performed using appropriate methods. A combination of transcriptome sequencing and real-time quantitative polymerase chain reaction (qRT-PCR) was used to measure the expression of functional genes that regulate the plasma membrane H+-ATPase activity in A. thaliana roots. Functional analysis was performed to understand how B. amyloliquefaciens regulates biological processes and metabolic pathways to strengthen A. thaliana resistance to alkaline stress. Here, we show that volatile organic compounds (VOCs) from B. amyloliquefaciens PDR1 promoted the growth and development of A. thaliana, enhanced the plasma membrane H+-ATPase activity, and affected ion absorption in Arabidopsis mots. Moreover, B. amyloliquefaciens PDR1 VOCs did not affect the expression of the gene coding for plasma membrane H+-ATPase, but affected the expression of genes regulating the activity of plasma membrane H+-ATPase. Our findings illuminate the mechanism by which B. amyloliquefaciens regulates the growth and alkaline stress resistance of A. thaliana, and lay a foundation for wide and efficient application for agricultural production and ecological protection.