PREVENTION OF DNA DAMAGE IN SPORES AND INVITRO BY SMALL, ACID-SOLUBLE PROTEINS FROM BACILLUS SPECIES
PREVENTION OF DNA DAMAGE IN SPORES AND INVITRO BY SMALL, ACID-SOLUBLE PROTEINS FROM BACILLUS SPECIES
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DOI：
10.1128/jb.175.5.1367-1374.1993
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发表时间：
1993-03-01
影响因子：
3.2
通讯作者：
SETLOW, P
中科院分区：
文献类型：
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作者：
FAIRHEAD, H;SETLOW, B;SETLOW, P
芽孢杆菌休眠孢子中的DNA被一组非特异性DNA结合蛋白饱和，称为α/β型小酸溶性孢子蛋白（SASP）。这些蛋白质在体内和体外改变DNA结构，提供孢子对紫外线的抗性。此外，热处理（例如，85 ℃，30分钟），其几乎不杀死B的野生型孢子。枯草芽孢杆菌杀灭>99%的缺乏大多数α/β型SASP的孢子（称为α-β-孢子）。在90 ℃、80 ℃、65 ℃、22 ℃和10 ℃下，发现野生型和α-β-孢子的存活率存在类似的巨大差异。经过热处理（85 ℃，30分钟）或长时间储存（22 ℃，6个月），α-β-孢子的杀灭率>99%，10 - 20%的存活者含有营养缺陷型或无孢子突变。然而，在85 ℃下加热30分钟的α-β-孢子释放的吡啶二羧酸并不比类似加热的野生型孢子（
The DNA in dormant spores of Bacillus species is saturated with a group of nonspecific DNA-binding proteins, termed alpha/beta-type small, acid-soluble spore proteins (SASP). These proteins alter DNA structure in vivo and in vitro, providing spore resistance to UV light. In addition, heat treatments (e.g., 85-degrees-C for 30 min) which give little killing of wild-type spores of B. subtilis kill >99% of spores which lack most alpha/beta-type SASP (termed alpha- beta- spores). Similar large differences in survival of wild-type and alpha- beta- spores were found at 90, 80, 65, 22, and 10-degrees-C. After heat treatment (85-degrees-C for 30 min) or prolonged storage (22-degrees-C for 6 months) that gave >99% killing of alpha- beta- spores, 10 to 20% of the survivors contained auxotrophic or asporogenous mutations. However, alpha- beta- spores heated for 30 min at 85-degrees-C released no more dipicolinic acid than similarly heated wild-type spores (